Human EGFR PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB10951P
Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.
1 Image
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Human EGFR PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGF R/ErbB1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ErbB2, rhErbB3, or rhErbB4 is observed.
Source
Monoclonal Rat IgG2A Clone # 423103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGF R/ErbB1
Leu25-Ser645
Accession # CAA25240
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of EGF R/ErbB1 antibody in A431 human epithelial carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Rat Anti-Human EGF R/ErbB1 PE-conjugated Monoclonal Antibody (Catalog # FAB10951P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EGFR

Epidermal Growth Factor Receptor (EGF R), also named erythroblastic leukemia viral oncogene homolog 1 (ErbB1), is a member of the type I receptor tyrosine kinase superfamily. The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain with two ligand binding cysteine rich domains, separated by a spacer region, and a cytoplasmic domain with a membrane proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGF R geneencodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha , in the absence of a coreceptor. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis (1 ‑ 3).

References
  1. Daly, R.J. (1999) Growth Factors, 16:255.
  2. Schlessinger, J. (2000) Cell. 103:211.
  3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

You must select a language.

x

Citations for Human EGFR PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
    Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
    Cellular Signalling, 2022-05-21;96(0):110360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach
    Authors: JJ Labisch, GP Wiese, K Barnes, F Bollmann, K Pflanz
    PLoS ONE, 2021-07-15;16(7):e0254739.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling
    Authors: JJ Labisch, F Bollmann, MW Wolff, K Pflanz
    Journal of biotechnology, 2020-12-07;326(0):11-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Cetuximab-induced natural killer cell cytotoxicity in head and neck squamous cell carcinoma cell lines: investigation of the role of cetuximab sensitivity and HPV status
    Authors: H Baysal, I De Pauw, H Zaryouh, J De Waele, M Peeters, P Pauwels, JB Vermorken, E Smits, F Lardon, J Jacobs, A Wouters
    Br. J. Cancer, 2020-06-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines
    Authors: I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters
    Mol Oncol, 2018-05-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Glioblastoma stem-like cell lines with either maintenance or loss of high-level EGFR amplification, generated via modulation of ligand concentration.
    Authors: Schulte A, Gunther HS, Martens T, Zapf S, Riethdorf S, Wulfing C, Stoupiec M, Westphal M, Lamszus K
    Clin. Cancer Res., 2012-02-07;18(7):1901-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human EGFR PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human EGFR PE-conjugated Antibody and earn rewards!

Have you used Human EGFR PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review