Human E-Cadherin Antibody

Catalog # Availability Size / Price Qty
MAB18381
MAB18381-SP
Detection of Human E‑Cadherin by Western Blot.
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (3)

Human E-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human E-Cadherin in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 180224
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
Asp155-Ile707
Accession # P12830
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
MCF‑7 human breast cancer cell line stained in buffer containing Ca2+ and Mg2+
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human E-Cadherin antibody by Western Blot. View Larger

Detection of Human E‑Cadherin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, BT-20 human breast cancer cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for E-Cadherin at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry E-Cadherin antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

E‑Cadherin in MCF‑7 Human Cell Line. E-Cadherin was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry E-Cadherin antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

E‑Cadherin in Human Stomach. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry E-Cadherin antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

E‑Cadherin in Human Skin. E-Cadherin was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human E-Cadherin Monoclonal Antibody (Catalog # MAB18381) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

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Citations for Human E-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Loss of polarity regulators initiates gasdermin-E-mediated pyroptosis in syncytiotrophoblasts
    Authors: Khushali Patel, Jasmine Nguyen, Sumaiyah Shaha, Amy Brightwell, Wendy Duan, Ashley Zubkowski et al.
    Life Science Alliance
  2. Tracking Drug‐Induced Epithelial–Mesenchymal Transition in Breast Cancer by a Microfluidic Surface‐Enhanced Raman Spectroscopy Immunoassay
    Authors: Zhen Zhang, Jing Wang, Karthik Balaji Shanmugasundaram, Belinda Yeo, Andreas Möller, Alain Wuethrich et al.
    Small
  3. EGFR activation-induced decreases in claudin1 promote MUC5AC expression and exacerbate asthma in mice
    Authors: Z Jia, K Bao, P Wei, X Yu, Y Zhang, X Wang, X Wang, L Yao, L Li, P Wu, W Yuan, S Wang, J Zheng, Y Hua, M Hong
    Mucosal Immunol, 2020-03-04;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates, Protein
    Applications: ICC, Western Blot
  4. Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
    Authors: Wada, Naohisa, Maeda, Hidefumi, Hasegawa, Daigaku, Gronthos, Stan, Bartold, Peter Ma, Menicanin, Danijela, Fujii, Shinsuke, Yoshida, Shinichi, Tomokiyo, Atsushi, Monnouchi, Satoshi, Akamine, Akifumi
    Stem Cells Dev, 2014-02-10;23(18):2225-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells.
    Authors: Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L
    Cell Transplant, 2011-12-21;21(6):1321-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
    Authors: Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S
    Blood, 2009-03-11;113(21):5157-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008-10-08;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity.
    Authors: Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K
    Cancer Res., 2004-03-15;64(6):2024-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

  1. Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    • The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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Reviews for Human E-Cadherin Antibody

Average Rating: 4 (Based on 3 Reviews)

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Human E-Cadherin Antibody
By Anonymous on 10/30/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: RT4 Urinary bladder transitional cell carcinoma cell line,RT4 Urinary bladder transitional cell carcinoma Species: Human

Human E-Cadherin Antibody
By Anonymous on 12/04/2016
Application: WB Sample Tested: Cell Lysates Species: Human

Expression of E-cadherin in different breast cancer cell lines.


Human E-Cadherin Antibody
By Anonymous on 07/19/2016
Application: Flow Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line Species: Human

Screen for the transfectant cells.