Human DUX4/DUX4c Antibody

Catalog #: MAB95351
5 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB95351-100
MAB95351-SP

Detection of Human DUX4/DUX4c by Western Blot.

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All DUX4/DUX4c Antibodies

Product Details

Citations (5)

Supplemental Products

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DUX4/DUX4c Antibody Summary

Species Reactivity

Human

Specificity

Detects human DUX4/DUX4c in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2143C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human DUX4 synthetic peptide containing sequences within amino acids 200-300 of the human DUX4 protein
Accession # Q9UBX2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DUX4/DUX4c by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with 100 ng/mL Doxycycline for overnight. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB95351) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DUX4/DUX4c

Human Double homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit has an open reading frame, DUX4, that contains two homeoboxes. Unregulated expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of the most prevalent genetic diseases of muscle.

Long Name

Double Homeobox 4/Double Homeobox 4c

Entrez Gene IDs

22947 (Human)

Alternate Names

DUX10; DUX4; DUX4/DUX4c; DUX4L

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human DUX4/DUX4c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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DUX4-r exerts a neomorphic activity that depends on GTF2I in acute lymphoblastic leukemia
Authors: Campolungo, D;Salomé, M;Biferali, B;Tascini, AS;Gabellini, D;
Science advances
Species: Human
Sample Types: Protein Extracts
Applications: Western Blot
Antiapoptotic Protein FAIM2 is targeted by miR-3202, and DUX4 via TRIM21, leading to cell death and defective myogenesis
Authors: HAN Soliman, EA Toso, IE Darwish, SM Ali, M Kyba
Cell Death & Disease, 2022-04-25;13(4):405.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Persistent Fibroadipogenic Progenitor Expansion Following Transient DUX4 Expression Provokes a Profibrotic State in a Mouse Model for FSHD
Authors: D Bosnakovsk, D Oyler, A Mitanoska, M Douglas, ET Ener, AS Shams, M Kyba
International Journal of Molecular Sciences, 2022-02-11;23(4):.
Species: Mouse
Sample Types: Whole Tissues
Applications: IHC
Transcriptional and cytopathological hallmarks of FSHD in chronic DUX4-expressing mice
Authors: D Bosnakovsk, AS Shams, C Yuan, MT da Silva, ET Ener, CW Baumann, AJ Lindsay, M Verma, A Asakura, DA Lowe, M Kyba
J. Clin. Invest., 2020-05-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Crystal Structure of the Double Homeodomain of DUX4 in Complex with DNA
Authors: JK Lee, D Bosnakovsk, EA Toso, T Dinh, S Banerjee, TE Bohl, K Shi, K Orellana, M Kyba, H Aihara
Cell Rep, 2018-12-11;25(11):2955-2962.e3.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot