Human DRAK1 Antibody

Catalog #: MAB4985 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4985
MAB4985-SP

Detection of Human DRAK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DRAK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DRAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) DRAK2, rhDAPK1, rhDAPK2, or rhDAPK3 is observed. In Western blots, no cross-reactivity with rhDRAK2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 684324

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DRAK1
Ala115-Glu313
Accession # Q9UEE5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DRAK1 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human DRAK1 Monoclonal Antibody (Catalog # MAB4985) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DRAK1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DRAK1

DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A) is a 55 kDa member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus, and initiates apoptosis. Human DRAK1 is 414 amino acids (aa) in length. It contains an N-terminal polyPro segment (aa 33-37) followed by a Ser/Thr kinase domain (aa 64-321) with a catalytic loop (aa 184-193). Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa 115-313, human DRAK1 shares 95% aa identity with canine DRAK1, and 65% aa identity with human DRAK2.

Long Name

DAP Kinase-related Apoptosis-inducing Protein Kinase 1

Entrez Gene IDs

9263 (Human)

Alternate Names

DAP kinase-related apoptosis-inducing protein kinase 1; DRAK1; DRAK1death-associated protein kinase-related 1; EC 2.7.11.1; serine/threonine kinase 17a (apoptosis-inducing); serine/threonine kinase 17a; serine/threonine-protein kinase 17A; STK17A