Human DNAM-1/CD226
Antibody

Catalog #: MAB10892 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10892-100
MAB10892-SP

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All DNAM-1/CD226 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNAM-1/CD226
Antibody Summary

Species Reactivity

Human

Specificity

Detects human DNAM-1/CD226 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2770C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human DNAM-1/CD226 protein
Glu19-Asn247
Accession # Q15762

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

Immersion fixed human NK cells and human peripheral blood mononuclear cells (PBMCs)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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DNAM-1/CD226 in Human NK cells. DNAM-1/CD226 was detected in immersion fixed human NK cells (positive staining) and U937 human histiocytic lymphoma cell line (negative staining) using Rabbit Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB10892) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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DNAM-1/CD226 in Human PBMCs. DNAM-1/CD226 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) (positive staining) and U937 human histiocytic lymphoma cell line (negative staining) using Rabbit Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB10892) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (2-6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7-10). Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung (11). CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation (12). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 13-15).

References

Zingoni, A. et al. (2013) Front. Immunol. 3:408.
Shibuya, A. et al. (1996) Immunity 4:573.
Bottino, C. et al. (2003) J. Exp. Med. 198:557.
Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
Morimoto, K. et al. (2007) Oncogene 27:264.
Chan, C.J. et al. (2014) Nat. Immunol. 15:431.
Shibuya, K. et al. (1999) Immunity 11:615.
Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Long Name

DNAX Accessory Molecule 1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Alternate Names

CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1