Human DcR3/TNFRSF6B Biotinylated Antibody

Catalog #: BAM2135 Datasheet / COA / SDS

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BAM2135

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DcR3/TNFRSF6B Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DcR3/TNFRSF6B in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF RII, rhTRAIL RI, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.

Source

Monoclonal Mouse IgG1 Clone # 229812

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human DcR3/TNFRSF6B Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human DcR3/TNFRSF6B Antibody (Catalog # MAB21351)

Standard: Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF (Catalog # 142-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: DcR3/TNFRSF6B

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

References

Pitti, R.M. et al. (1998) Nature 396:699.
Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.

Long Name

Decoy Receptor 3

Entrez Gene IDs

8771 (Human); 107126607 (Cynomolgus Monkey)

Alternate Names

DcR3; DCR3Decoy receptor for Fas ligand; Decoy receptor 3; M68; M68DJ583P15.1.1; TNFRSF6B; TR6; TR6DcR3; tumor necrosis factor receptor superfamily member 6B; tumor necrosis factor receptor superfamily, member 6b, decoy