Human DC-STAMP Antibody

Catalog #: MAB7824 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7824
MAB7824-SP

Detection of DC-STAMP in Human Mature Dendritic Cells by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DC-STAMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human DC-STAMP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DC-STAMP is observed.

Source

Monoclonal Mouse IgG_2B Clone # 788524

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DC-STAMP
Asp314-Thr376
Accession # Q9H295

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of DC-STAMP in Human Mature Dendritic Cells by Flow Cytometry. Human mature dendritic cells derived from CD14⁺Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 6 days then treated with 20 ng/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours were stained with Mouse Anti-Human CD83 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1774F) and either (A) Mouse IgG_2BIsotype Control (Catalog # MAB0041) or (B) Mouse Anti-Human DC-STAMP Monoclonal Antibody (Catalog # MAB7824) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-STAMP

DC-STAMP, also known as TM7SF4, is an approximately 50 kDa glycoprotein with seven transmembrane segments. It is expressed on the surface of dendritic cells and monocytes as well as on osteoclasts and their progenitors. DC-STAMP binds CTGF/CCN2 and cooperates with TRANCE/RANK L for inducing osteoclast differentiation and fusion into multinucleated cells. It contains a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and associates with FC gamma RIII/CD16. Within aa 314-376, human DC-STAMP shares approximately 75% aa sequence identity with mouse and rat DC-STAMP.

Long Name

Dendritic Cell-specific Transmembrane Protein

Entrez Gene IDs

81501 (Human); 75766 (Mouse); 299889 (Rat)

Alternate Names

DCSTAMP; DC-STAMP; dendritic cell-specific transmembrane protein; FIND; IL-4-induced Protein; TM7SF4; transmembrane 7 superfamily member 4