Human DC-SIGNR/CD299 PE-conjugated Antibody
Human DC-SIGNR/CD299 PE-conjugated Antibody Summary
Accession # Q9H2X3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DC‑SIGNR/CD299 in 3T3 Mouse Cell Line Transfected with Human DC-SIGNR/CD299 by Flow Cytometry. 3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR/CD299 was stained with Mouse Anti-Human DC-SIGNR/CD299 PE-conjugated Monoclonal Antibody (Catalog # FAB162P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DC-SIGNR/CD299
Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1-4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).
- Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
- Pohlmann, S. et al. (2003) J. Virol. 77:4070.
- Simmons, L.G. et al. (2003) J. Virol. 77:1337.
- Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
- Feinberg, H. et al. (2001) Science 294:2163.
Product Datasheets
Citations for Human DC-SIGNR/CD299 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Relationship between the Number of Repeats in the Neck Regions of L-SIGN and Augmented Virus Replication and Immune Responses in Dengue Hemorrhagic Fever
Authors: Liu, KS;Chen, PM;Wang, L;Lee, IK;Yang, KD;Chen, RF;
International journal of molecular sciences
Species: Human
Sample Types: Transfected Whole Cells
Applications: Flow Cytometry -
Identification of HIV transmitting CD11c+ human epidermal dendritic cells
Authors: KM Bertram, RA Botting, H Baharlou, JW Rhodes, H Rana, JD Graham, E Patrick, J Fletcher, TM Plasto, NR Truong, C Royle, CM Doyle, O Tong, N Nasr, L Barnouti, MP Kohout, AJ Brooks, MP Wines, P Haertsch, J Lim, MP Gosselink, G Ctercteko, JD Estes, MJ Churchill, PU Cameron, E Hunter, MA Haniffa, AL Cunningham, AN Harman
Nat Commun, 2019-06-21;10(1):2759.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
J. Leukoc. Biol, 2017-03-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mechanism and significance of cell type-dependent neutralization of flaviviruses.
Authors: Mukherjee S, Dowd K, Manhart C, Ledgerwood J, Durbin A, Whitehead S, Pierson T
J Virol, 2014-04-16;88(13):7210-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
J. Immunol., 2002-10-15;169(8):4172-82.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry
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Expression of DC-SIGNR on human monocyte-derived macrophages. The cells were treated with 10 μg/ml of the DC-SIGNR PE antibody (catalogue # FAB162P) (blue) or mouse IgG2b PE isotype control antibody (Red)
