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Human DC-SIGNR/CD299 Antibody

Catalog #: MAB162
17 Citations Datasheet / COA / SDS
Clone 120604 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB162
MAB162-SP
Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry.
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Citations (17)
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Human DC-SIGNR/CD299 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGNR/CD299.
Source
Monoclonal Mouse IgG2B Clone # 120604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
Accession # Q9H2X3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Adhesion Blockade
The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 2.5 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DC-SIGNR/CD299 antibody in Human DC-SIGNR/CD299 Transfected 3T3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC-SIGNR/CD299 and DC-SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Immunocytochemistry/ Immunofluorescence Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human DC-SIGNR/CD299/CLEC4M by Immunocytochemistry/Immunofluorescence In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGNR/CD299

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

References
  1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
  2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
  3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
  4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
  5. Feinberg, H. et al. (2001) Science 294:2163.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2
Entrez Gene IDs
10332 (Human)
Alternate Names
CD209L; CD209L1; CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2; CD299 antigenMGC129964; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DC-SIGN2CD209 antigen-like protein 1; DCSIGNR; DC-SIGNR; DC-SIGNRDC-SIGN-related protein; DCSIGNRMGC47866; Dendritic cell-specific ICAM-3-grabbing non-integrin 2; HP10347; liver/lymph node-specific ICAM-3 grabbing non-integrin; Liver/lymph node-specific ICAM-3-grabbing non-integrin; LSIGN; L-SIGN; LSIGNC-type lectin domain family 4 member M; mannose binding C-type lectin DC-SIGNR

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Citations for Human DC-SIGNR/CD299 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Envelope Protein Glycosylation Mediates Zika Virus Pathogenesis
    Authors: Derek L. Carbaugh, Ralph S. Baric, Helen M. Lazear
    Journal of Virology
  2. A Single-Cell Transcriptional Roadmap of the Mouse and Human Lymph Node Lymphatic Vasculature
    Authors: Menglan Xiang, Rubén Adrián Grosso, Akira Takeda, Junliang Pan, Tove Bekkhus, Kevin Brulois et al.
    Frontiers in Cardiovascular Medicine
  3. Human Monoclonal Antibodies against NS1 Protein Protect against Lethal West Nile Virus Infection
    Authors: AW Wessel, MP Doyle, TB Engdahl, J Rodriguez, JE Crowe, MS Diamond
    MBio, 2021-10-12;12(5):e0244021.
    Species: Human
    Sample Types: Whole Cells
  4. L-SIGN is a receptor on liver sinusoidal endothelial cells for SARS-CoV-2 virus
    Authors: Y Kondo, JL Larabee, L Gao, H Shi, B Shao, CM Hoover, JM McDaniel, YC Ho, R Silasi-Man, SA Archer-Har, P Azadi, RS Srinivasan, AR Rezaie, A Borczuk, JC Laurence, F Lupu, J Ahamed, RP McEver, JF Papin, Z Yu, L Xia
    JCI Insight, 2021-07-22;6(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  5. Dengue viruses infect human megakaryocytes, with probable clinical consequences
    Authors: MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse
    PLoS Negl Trop Dis, 2019-11-25;13(11):e0007837.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The endothelial cell receptor stabilin-2 regulates VWF-FVIII complex half-life and immunogenicity
    Authors: LL Swystun, JD Lai, C Notley, I Georgescu, AS Paine, J Mewburn, K Nesbitt, K Schledzews, C Géraud, J Kzhyshkows, S Goerdt, W Hopman, RR Montgomery, PD James, D Lillicrap
    J. Clin. Invest., 2018-08-20;128(9):4057-4073.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2011-11-16;86(3):1339-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
    Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
    J. Virol., 2009-08-19;83(21):10908-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
    Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
    J. Hepatol., 2009-07-30;51(4):734-49.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets.
    Authors: Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S
    J. Virol., 2006-09-01;80(18):8951-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. L-SIGN (CD209L) isoforms differently mediate trans-infection of hepatoma cells by hepatitis C virus pseudoparticles.
    Authors: Falkowska E, Durso R, Gardner J, Cormier E, Arrigale R, Ogawa R, Donovan G, Maddon P, Olson W, Dragic T
    J Gen Virol, 2006-09-01;87(0):2571-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
    Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH
    Am. J. Pathol., 2006-07-01;169(1):200-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  14. Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation.
    Authors: Ludwig IS, Lekkerkerker AN, Depla E, Bosman F, Musters RJ, Depraetere S, van Kooyk Y, Geijtenbeek TB
    J. Virol., 2004-08-01;78(15):8322-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors
    Authors: Lei Guo, Yan Liang, Heng Li, Huiwen Zheng, Zening Yang, Yanli Chen et al.
    iScience
  16. A protective human monoclonal antibody targeting the West Nile virus E protein preferentially recognizes mature virions.
    Authors: Goo L, Debbink K, Kose N et al.
    Nat Microbiol.
  17. CLEC4M-positive and CD81-negative Huh7 cells are not susceptible to JFH-1 HCVcc infection but mediate transinfection
    Authors: Mariko Ishibashi, Naoko Morita, Chisato Nomura-Kawaguchi, Yohko Shimizu, Takaji Wakita, Mariko Esumi
    Archives of Virology

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