Human Cyclin D1 Antibody

Catalog #: MAB43142 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB43142-100
MAB43142-SP

Cyclin D1 in WM-115 and Daudi Human Cell Lines.

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All Cyclin D1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cyclin D1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cyclin D1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 328311

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Cyclin D1 in WM-115 and Daudi Human Cell Lines. Cyclin D1 was detected in immersion fixed WM-115 human malignant melanoma cell line (positive stain) and Daudi human Burkitt's lymphoma cell line (negative stain) using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB43142) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin D1

Cyclin D1 (CCND1) is a 36 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.

Entrez Gene IDs

595 (Human); 12443 (Mouse); 58919 (Rat)

Alternate Names

B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31