Home / CXCR7/RDC-1 / Human CXCR7/RDC-1 Antibody

Human CXCR7/RDC-1 Antibody

Catalog #: MAB42273
36 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB42273
MAB42273-SP
Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry.
4 Images
Product Details
Citations (36)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR7/RDC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.
Source
Monoclonal Mouse IgG1 Clone # 11G8
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CXCR7 encoding plasmid
Accession # P25106
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR7/RDC‑1 antibody in MCF‑7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Immunohistochemistry CXCR7/RDC-1 antibody in Human Squamous Cell Carcinoma by Immunohistochemistry (IHC-P). View Larger

CXCR7/RDC‑1 in Human Squamous Cell Carcinoma. CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Functional Detection of Human CXCR7/RDC-1 by Functional View Larger

Detection of Human CXCR7/RDC-1 by Functional CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. HUVECs were fixed and stained with antibodies against CXCR4 (purple), CXCR7 (blue) and F-Actin (red). The micro stripes of fluorescein-conjugated BSA (A) or CXCL12 (H) are shown. CXCR4 (B), CXCR7 (C) and F-Actin (D) in HUVECs cultured on BSA stripes stayed in the resting state, while the polarization of CXCR4 (arrow: I), CXCR7 (arrow: J) and F-Actin (arrow: K) was observed in HUVECs cultured on CXCL12 stripes for 5 min. Moreover, the HUVECs in the BSA control group showed no morphological changes (E–G). The CXCL12 group HUVECs shows polarization towards the CXCL12 stripe (L–N). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28811579), licensed under a CC-BY license. Not internally tested by R&D Systems.

Functional Detection of Human CXCR7/RDC-1 by Functional View Larger

Detection of Human CXCR7/RDC-1 by Functional CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. HUVECs were fixed and stained with antibodies against CXCR4 (purple), CXCR7 (blue) and F-Actin (red). The micro stripes of fluorescein-conjugated BSA (A) or CXCL12 (H) are shown. CXCR4 (B), CXCR7 (C) and F-Actin (D) in HUVECs cultured on BSA stripes stayed in the resting state, while the polarization of CXCR4 (arrow: I), CXCR7 (arrow: J) and F-Actin (arrow: K) was observed in HUVECs cultured on CXCL12 stripes for 5 min. Moreover, the HUVECs in the BSA control group showed no morphological changes (E–G). The CXCL12 group HUVECs shows polarization towards the CXCL12 stripe (L–N). Scale bar: 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28811579), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Entrez Gene IDs
57007 (Human); 12778 (Mouse); 84348 (Rat)
Alternate Names
ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human CXCR7/RDC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Partial agonist activity of alpha 1-adrenergic receptor antagonists for chemokine (C-X-C motif) receptor 4 and atypical chemokine receptor 3
    Authors: Xianlong Gao, Hazem Abdelkarim, Lauren J. Albee, Brian F. Volkman, Vadim Gaponenko, Matthias Majetschak
    PLOS ONE
  2. Effects of Small Molecule Ligands on ACKR3 Receptors
    Authors: Brittany E. Hopkins, Ikuo Masuho, Dongjun Ren, Iredia D. Iyamu, Wei Lv, Neha Malik et al.
    Molecular Pharmacology
  3. Alternative splicing of helicase-like transcription factor (Hltf): Intron retention-dependent activation of immune tolerance at the feto-maternal interface
    Authors: Gurvinder Kaur, Rebecca A. Helmer, Lisa A. Smith, Raul Martinez-Zaguilan, Jannette M. Dufour, Beverly S. Chilton
    PLOS ONE
  4. CXCR4 expression affects overall survival of HCC patients whereas CXCR7 expression does not
    Authors: Maria Neve Polimeno, Caterina Ierano, Crescenzo D'Alterio, Nunzia Simona Losito, Maria Napolitano, Luigi Portella et al.
    Cellular & Molecular Immunology
  5. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling
    Authors: ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh
    Cell Death Dis, 2020-05-04;11(5):307.
  6. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  7. Differential expression of CXCR4 and CXCR7 with various stem cell markers in paired human primary and recurrent glioblastomas
    Authors: CHARLOTTE FLÜH, KIRSTEN HATTERMANN, H. MAXIMILIAN MEHDORN, MICHAEL SYNOWITZ, JANKA HELD-FEINDT
    International Journal of Oncology
  8. Disclosing the CXCR4 Expression in Lymphoproliferative Diseases by Targeted Molecular Imaging
    Authors: Hans Jürgen Wester, Ulrich Keller, Margret Schottelius, Ambros Beer, Kathrin Philipp-Abbrederis, Frauke Hoffmann et al.
    Theranostics
  9. The role of PKC and PKD in CXCL12 and CXCL13 directed malignant melanoma and acute monocytic leukemic cancer cell migration
    Authors: Hamshaw, I;Ellahouny, Y;Malusickis, A;Newman, L;Ortiz-Jacobs, D;Mueller, A;
    Cellular signalling
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. CXCR4-CXCL12-CXCR7 and PD-1/PD-L1 in Pancreatic Cancer: CXCL12 Predicts Survival of Radically Resected Patients
    Authors: C D'Alterio, A Giardino, G Scognamigl, G Butturini, L Portella, G Guardascio, I Frigerio, M Montella, S Gobbo, G Martignoni, V Napolitano, F De Vita, F Tatangelo, R Franco, S Scala
    Cells, 2022-10-22;11(21):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Biological Role of Tumor/Stromal CXCR4-CXCL12-CXCR7 in MITO16A/MaNGO-OV2 Advanced Ovarian Cancer Patients
    Authors: C D'Alterio, A Spina, L Arenare, P Chiodini, M Napolitano, F Galdiero, L Portella, V Simeon, S Signoriell, F Raspaglies, D Lorusso, C Pisano, N Colombo, GF Zannoni, NS Losito, R De Cecio, G Scognamigl, D Califano, D Russo, V Tuninetti, MC Piccirillo, P Gargiulo, F Perrone, S Pignata, S Scala
    Cancers, 2022-04-06;14(7):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
    Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
    International Journal of Molecular Sciences, 2022-01-14;23(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. The atypical chemokine receptor ACKR3/CXCR7 is a broad-spectrum scavenger for opioid peptides
    Authors: M Meyrath, M Szpakowska, J Zeiner, L Massotte, MP Merz, T Benkel, K Simon, J Ohnmacht, JD Turner, R Krüger, V Seutin, M Ollert, E Kostenis, A Chevigné
    Nat Commun, 2020-06-19;11(1):3033.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking
    Authors: N Pluchino, R Mamillapal, S Shaikh, S Habata, A Tal, M Gaye, HS Taylor
    J. Cell. Mol. Med., 2020-01-06;24(4):2464-2474.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells
    Authors: C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala
    Cell Death Dis, 2019-07-22;10(8):562.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer
    Authors: S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu
    Cancer Res., 2019-04-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. CXCR7 promotes melanoma tumorigenesis via Src kinase signaling
    Authors: S Xu, J Tang, C Wang, J Liu, Y Fu, Y Luo
    Cell Death Dis, 2019-02-25;10(3):191.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. The Role of CXCL12 Axis in Lung Metastasis of Colorectal Cancer
    Authors: M Wang, X Yang, M Wei, Z Wang
    J Cancer, 2018-10-10;9(21):3898-3903.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration
    Authors: E Willems, M Dedobbelee, M Digregorio, A Lombard, N Goffart, PN Lumapat, J Lambert, P Van den Ac, M Szpakowska, A Chevigné, F Scholtes, B Rogister
    Oncogene, 2018-08-06;0(0):.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Cell Selection
  20. G-Protein-Coupled Receptor CXCR7 Is Overexpressed in Human and Murine Endometriosis
    Authors: N Pluchino, R Mamillapal, I Moridi, R Tal, HS Taylor
    Reprod Sci, 2018-03-27;0(0):1933719118766.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. CXCR7/ACKR3-targeting ligands interfere with X7 HIV-1 and HIV-2 entry and replication in human host cells
    Authors: T D'huys, S Claes, T Van Loy, D Schols
    Heliyon, 2018-03-01;4(3):e00557.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Inhibition
  22. Ligand-specific conformational transitions and intracellular transport required for atypical chemokine receptor 3-mediated chemokine scavenging
    Authors: N Montpas, G St-Onge, N Nama, D Rhainds, B Benredjem, M Girard, G Hickson, V Pons, N Heveker
    J. Biol. Chem., 2017-11-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Effects of cognate, non-cognate and synthetic CXCR4 and ACKR3 ligands on human lung endothelial cell barrier function
    Authors: YH Cheng, JM Eby, HM LaPorte, BF Volkman, M Majetschak
    PLoS ONE, 2017-11-10;12(11):e0187949.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  24. CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
    Authors: M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang
    Sci Rep, 2017-08-15;7(1):8289.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Cathepsin K cleavage of SDF-1? inhibits its chemotactic activity towards glioblastoma stem-like cells
    Authors: Vashendriya V V Hira
    Biochim. Biophys. Acta, 2016-12-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. The CXCR4/CXCR7/CXCL12 Axis Is Involved in a Secondary but Complex Control of Neuroblastoma Metastatic Cell Homing.
    Authors: Muhlethaler-Mottet A, Liberman J, Ascencao K, Flahaut M, Balmas Bourloud K, Yan P, Jauquier N, Gross N, Joseph J
    PLoS ONE, 2015-05-08;10(5):e0125616.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4.
    Authors: Walsh T, Harper M, Poole A
    Cell Signal, 2014-10-05;27(1):37-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
    Authors: Liu Y, Carson-Walter E, Walter K
    PLoS ONE, 2014-08-01;9(8):e103938.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  29. COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
    Authors: Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F
    BMC Cancer, 2014-06-06;14(0):407.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  30. Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats.
    Authors: Walters, M J, Ebsworth, K, Berahovich, R D, Penfold, M E T, Liu, S-C, Al Omran, R, Kioi, M, Chernikova, S B, Tseng, D, Mulkearns-Hubert, E E, Sinyuk, M, Ransohoff, R M, Lathia, J D, Karamchandani, J, Kohrt, H E K, Zhang, P, Powers, J P, Jaen, J C, Schall, T J, Merchant, M, Recht, L, Brown, J M
    Br J Cancer, 2014-01-14;110(5):1179-88.
    Species: Human, Rat
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  31. Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
    Authors: Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S
    Nature, 2013-11-20;505(7481):97-102.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot
  32. Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
    Authors: Liberman J, Sartelet H, Flahaut M, Muhlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph J, Gross N
    PLoS ONE, 2012-08-20;7(8):e43665.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Ubiquitination of CXCR7 controls receptor trafficking.
    Authors: Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R
    PLoS ONE, 2012-03-23;7(3):e34192.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, ICC
  34. CXCR7 functions as a scavenger for CXCL12 and CXCL11.
    Authors: Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M
    PLoS ONE, 2010-02-11;5(2):e9175.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Commercially available antibodies directed against alpha -adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments
    Authors: Abhishek Tripathi, Vadim Gaponenko, Matthias Majetschak
    Naunyn-Schmiedeberg's Archives of Pharmacology
  36. New Insights into Mechanisms and Functions of Chemokine (C-X-C Motif) Receptor 4 Heteromerization in Vascular Smooth Muscle.
    Authors: Evans AE, Tripathi A, LaPorte HM et al.
    Int. J. Mol. Sci.

FAQs

  1. What light chain subclass is MAB42273 and MAB42273R? 

    • MAB42273 and MAB42273R have a kappa light chain. 

View all Antibody FAQs
Loading...

Reviews for Human CXCR7/RDC-1 Antibody

There are currently no reviews for this product. Be the first to review Human CXCR7/RDC-1 Antibody and earn rewards!

Have you used Human CXCR7/RDC-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review