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Human CXCR6 PE-conjugated Antibody

Catalog #: FAB699P
17 Citations Datasheet / COA / SDS
Clone 56811 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB699P-025
FAB699P-100
Detection of CXCR6 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR6 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR6 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 56811
Purification
Protein A or G purified from ascites
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR6
Met1-Leu342
Accession # O00574
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and HEK293 cells transfected with Human CXCR6 and eGFP vs irrelevant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR6 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CXCR6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 150 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days were stained with Mouse Anti-Human CXCR6 PE-conjugated Monoclonal Antibody (Catalog # FAB699P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (IC0041P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CXCR6 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human CXCR6 and eGFP (A) vs irrelevant and eGFP (B) were stained with Mouse Anti-Human CXCR6 PE‑conjugated Monoclonal Antibody (Catalog # FAB699P) at 37°C. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human CXCR6 by Flow Cytometry View Larger

Detection of Human CXCR6 by Flow Cytometry CXCR6 and CXCL16 expression in LuCa cell lines(A) mRNA levels by semiquantitative RT-PCR. The copies of CXCR6 and CXCL16 transcripts are expressed relative to copies of 18S rRNA. Values are mean ± SEM from 3 independent experiments. *p ≤ 0.05, ***p ≤ 0.001 as compared to SCC (NCI-H520). (B) Flow cytometry analysis of CXCR6 and trans-membrane CXCL16 in (i) - SCC (NCI-H520) cells and (ii) - AC (NCI-H2126) cells. Grey dots represent isotype controls for PE and APC antibody and black dots represent CXCR6-PE and CXCL16-APC in SCC (NCI-H520) and AC (NCI-H2126). CXCR6 and CXCL16 both are expressed on ~82.9% (Q2) SCC cells; 5.41% (Q1) express only CXCR6 and only CXCL16 is expressed by 9.08% (Q3) of SCC cells. In AC cells, 61.7% of the population express both receptor and ligand (Q2) whereas, 11.1 (Q1) and 16.2% (Q3) cells express only CXCR6 and CXCL16, respectively. c) Levels of soluble CXCL16 in LuCa supernatant. Values are mean ± SEM from 3 independent experiments. ***p ≤ 0.0001 compared to NCI-H520. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Entrez Gene IDs
10663 (Human); 80901 (Mouse); 100124593 (Rat)
Alternate Names
Bonzo; BONZOG-protein coupled receptor bonzo; CD186 antigen; CD186; CDw186; chemokine (C-X-C motif) receptor 6; C-X-C chemokine receptor type 6; CXCR6; CXC-R6; CXCR-6; G protein-coupled receptor; STRL33; STRL33G-protein coupled receptor STRL33; TYMSTRCD186

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Citations for Human CXCR6 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Human liver infiltrating gamma δ T cells are composed of clonally expanded circulating and tissue-resident populations
    Authors: Stuart Hunter, Carrie R. Willcox, Martin S. Davey, Sofya A. Kasatskaya, Hannah C. Jeffery, Dmitriy M. Chudakov et al.
    Journal of Hepatology
  2. The DRF motif of CXCR6 as chemokine receptor adaptation to adhesion
    Authors: A Koenen, A Babendreye, J Schumacher, T Pasqualon, N Schwarz, A Seifert, X Deupi, A Ludwig, D Dreymuelle
    PLoS ONE, 2017-03-07;12(3):e0173486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Detection of Primary T Cell Responses to Drugs and Chemicals in HLA-Typed Volunteers: Implications for the Prediction of Drug Immunogenicity
    Authors: Lee Faulkner
    Toxicol. Sci, 2016-09-16;154(2):416-429.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways.
    Authors: Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D
    J Autoimmun, 2015-08-30;66(0):40-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
    Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
    PLoS ONE, 2015-03-16;10(3):e0120069.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Invariant natural killer T cells act as an extravascular cytotoxic barrier for joint-invading Lyme Borrelia.
    Authors: Lee, Woo-Yong, Sanz, Maria-Je, Wong, Connie H, Hardy, Pierre-O, Salman-Dilgimen, Aydan, Moriarty, Tara J, Chaconas, George, Marques, Adriana, Krawetz, Roman, Mody, Christop, Kubes, Paul
    Proc Natl Acad Sci U S A, 2014-09-09;111(38):13936-41.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014-06-04;9(6):e99056.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
    Authors: Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
    Clin. Exp. Immunol., 2010-11-19;163(2):215-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
    Authors: Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
    Blood, 2010-11-17;117(4):1250-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection.
    Authors: Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J
    J. Immunol., 2009-12-16;184(2):984-92.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Expression of the CXCR6 on polymorphonuclear neutrophils in pancreatic carcinoma and in acute, localized bacterial infections.
    Authors: Gaida MM, Gunther F, Wagner C, Friess H, Giese NA, Schmidt J, Hansch GM, Wente MN
    Clin. Exp. Immunol., 2008-09-05;154(2):216-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. CXCR6-CXCL16 interaction in the pathogenesis of Juvenile Idiopathic Arthritis.
    Authors: Martini G, Cabrelle A, Calabrese F, Carraro S, Scquizzato E, Teramo A, Facco M, Zulian F, Agostini C
    Clin. Immunol., 2008-08-28;129(2):268-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  14. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007-09-15;179(6):4272-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. CC chemokine receptor 5 and CXC chemokine receptor 6 expression by lung CD8+ cells correlates with chronic obstructive pulmonary disease severity.
    Authors: Freeman CM, Curtis JL, Chensue SW
    Am. J. Pathol., 2007-07-19;171(3):767-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Cross-Talk Between circRNAs and mRNAs Modulates MiRNA-mediated Circuits and Affects Melanoma Plasticity
    Authors: Maria Rita Fumagalli, Maria Chiara Lionetti, Stefano Zapperi, Caterina A. M. La Porta
    Cancer Microenvironment
  17. CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
    Authors: Lepore F, D'Alessandro G, Antonangeli F et al.
    Front Immunol

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