Human CXCL5/ENA-78 Antibody

Name: Human CXCL5/ENA-78 Antibody
Brand: R&D Systems
SKU: MAB654
Rating: 4 (1 reviews)

Catalog #: MAB654
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB654-SP
MAB654-500
MAB654-100

Detection of CXCL5/ENA‑78 in Human PBMCs by Flow Cytometry.

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All CXCL5/ENA-78 Antibodies

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Citations (2)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL5/ENA-78 Antibody Summary

Specificity

Detects human CXCL5/ENA-78 in ELISAs. Does not cross-react with recombinant human CXCL1, 2, or 3.

Source

Monoclonal Mouse IgG₁ Clone # 33170

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CXCL5/ENA-78
Ala37-Asn114
Accession # P42830

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Human CXCL5/ENA-78 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CXCL5/ENA‑78 Biotinylated Antibody (Catalog # BAF254)

Standard: Recombinant Human CXCL5/ENA-78 Protein (Catalog # 254-XB)

Neutralization

Measured by its ability to neutralize CXCL5/ENA‑78-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.65-5 µg/mL in the presence of 30 ng/mL Recombinant Human CXCL5/ENA‑78.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of CXCL5/ENA-78 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of CXCL5/ENA‑78 in Human PBMCs by Flow Cytometry. Human peripheral blood mononculear cells (PBMCs) either treated with LPS (filled histogram) or untreated (open histogram) were stained with Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Neutralization

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Chemotaxis Induced by CXCL5/ENA-78 and Neutralization by Human CXCL5/ENA-78 Antibody. Recombinant Human CXCL5/ENA-78 (254-XB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL5/ ENA-78 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL5/ENA-78 Monoclonal Antibody (Catalog # MAB654). The ND₅₀is typically 0.65-5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL5/ENA-78

CXCL5, also known as ENA-78, is a CXC chemokine that plays a role in inflammatory responses and angiogenesis.

Entrez Gene IDs

6374 (Human)

Alternate Names

chemokine (C-C motif) ligand 5; C-X-C motif chemokine 5; CXCL5; CXCL5/ENA-78; ENA78; ENA-78; ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5ENA78

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CXCL5/ENA-78 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
Cell reports
Species: Murine polyomavirus strain A3
Sample Types: Whole Cells
Applications: Mass Cytometry
Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
Authors: Edwards MR, Johnson MW, Johnston SL
Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development