At what concentration do we use GRO-alpha, GRO-beta, and GRO-gamma for neutralization in bioassay with Catalog # MAB276?

Name: Human CXCL1/2/3/GRO Pan Specific Antibody
Brand: R&D Systems
SKU: MAB276
Price: 459 USD

Recombinant Human GRO-beta (Catalog # 176-GB) is used at 6 ng/mL, rhGRO-alpha (Catalog # 275-GR) is used at 10 ng/mL, and rhGRO-gamma (Catalog # 277-GG) is used at 20 ng/mL.

Human CXCL1/2/3/GRO Pan Specific Antibody

Catalog #: MAB276
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB276-SP
MAB276-100
MAB276-500

Chemotaxis Induced by CXCL2/GRO beta and Neutralization by Human CXCL1/2/3/GRO Antibody.

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Citations (11)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCL1/2/3/GRO Pan Specific Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL1/2/3 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 31716

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CXCL2
Ala35-Asn107
Accession # P19875

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR)
Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 276-GB)
Recombinant Human CXCL3/GRO gamma /CINC-2/DCIP-1 (Catalog # 277-GG)

Neutralization

Measured by its ability to neutralize CXCL1/2/3/GRO-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 µg/mL, 0.15-0.75µg/mL, and 0.1-0.5 µg/mL for Recombinant Human (rh) CXCL1, rhCXCL2, and rhCXCL3, respectively.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL2/GRO beta and Neutralization by Human CXCL1/2/3/GRO Antibody. Recombinant Human CXCL2/GRO beta (Catalog # 176-GB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL2/GRO beta (6 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB276). The ND₅₀ is typically 0.15-0.75 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL1/2/3/GRO

CXCL1, CXCL2 (MIP-2 alpha ), and CXCL3 (MIP-2 beta ) are members of the alpha (C-X-C) subfamily of chemokines and are now designated GRO alpha, GRO beta, and GRO gamma, respectively. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.

Alternate Names

CXCL1/2/3; FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CXCL1/2/3/GRO Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Overexpression of B7‑H4 promotes renal cell carcinoma progression by recruiting tumor‑associated neutrophils via upregulation of CXCL8
Authors: Anqi Li, Ningyue Zhang, Zhiming Zhao, Yali Chen, Liang Zhang
Oncology Letters
Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein C expression in alveolar epithelial cells
Authors: Bijesh Puthusseri, Amarnath Marudamuthu, Nivedita Tiwari, Jian Fu, Steven Idell, Sreerama Shetty
American Journal of Physiology-Lung Cellular and Molecular Physiology
A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer
Authors: Ming-Na Zhao, Ling-Fei Zhang, Zhen Sun, Li-Hua Qiao, Tao Yang, Yi-Zhe Ren et al.
Cell Death & Disease
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
Psychoneuroendocrinology, 2018-07-13;9(1):2705.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Gene expression profiling of primary human type I alveolar epithelial cells exposed to Bacillus anthracis spores reveals induction of neutrophil and monocyte chemokines
Authors: JL Booth, ES Duggan, VI Patel, W Wu, DM Burian, DC Hutchings, VL White, KM Coggeshall, MG Dozmorov, JP Metcalf
Microb. Pathog., 2018-04-25;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization
Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
J Immunol, 2014-05-28;193(1):234-43.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
CXCR2-driven ovarian cancer progression involves upregulation of proinflammatory chemokines by potentiating NF-kappaB activation via EGFR-transactivated Akt signaling.
Authors: Dong Y, Kabir S, Lee E, Son D
PLoS ONE, 2013-12-20;8(12):e83789.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
J. Immunol., 2010-03-24;184(0):5263-70.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development
L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
Atherosclerosis, 2006-10-04;192(1):56-66.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells.
Authors: Hagiwara H, Mitsumata M, Yamane T
Circulation, 1998-12-08;98(23):2584-90.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot

FAQs

At what concentration do we use GRO-alpha, GRO-beta, and GRO-gamma for neutralization in bioassay with Catalog # MAB276?
- Recombinant Human GRO-beta (Catalog # 176-GB) is used at 6 ng/mL, rhGRO-alpha (Catalog # 275-GR) is used at 10 ng/mL, and rhGRO-gamma (Catalog # 277-GG) is used at 20 ng/mL.

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