Human CX3CL1/Fractalkine APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC365A
Detection of CX3CL1/Fractalkine in NS0 Mouse Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry.
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Human CX3CL1/Fractalkine APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 51637
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
Gln25-Arg339 (Ser199Asn) (predicted)
Accession # P78423
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CX3CL1/Fractalkine antibody in NS0 Mouse Cell Line Transfected with His-tagged Human CX3CL1 antibody by Flow Cytometry. View Larger

Detection of CX3CL1/Fractalkine in NS0 Mouse Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry. NS0 mouse myeloma cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag PE-conjugated Monoclonal Antibody (Catalog # IC050P) and either (A) Mouse Anti-Human CX3CL1/Fractalkine APC-conjugated Monoclonal Antibody (Catalog # IC365A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine and Neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

References
  1. Pan, Y. et al. (1997) Nature 387:611.
  2. Bazan, J.F. et al. (1997) Nature 385:640.
  3. Mackay, C.R. (1997) Current Biology 7:R384.
Entrez Gene IDs
6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)
Alternate Names
ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1

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Citation for Human CX3CL1/Fractalkine APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dorsal Root Ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain
    Authors: S Oggero, C Cecconello, R Silva, L Zeboudj, G Sideris-La, M Perretti, M Malcangio
    Brain, Behavior, and Immunity, 2022-09-14;106(0):289-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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