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Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody

Catalog #: MAB9928 Datasheet / COA / SDS
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9928-100
MAB9928-SP
Detection of CTLA-4 in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP by Flow Cytometry.
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Human CTLA-4 (Research Grade Ipilimumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human CTLA-4 based on Ipilimumab therapeutic antibody.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Ipilimumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu111
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human CTLA-4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CTLA-4 antibody in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP antibody by Flow Cytometry. View Larger

Detection of CTLA-4 in HEK293 Human cell line Transfected with Human CTLA-4 and eGFP by Flow Cytometry. HEK293 Human cell line transfected with (A) Human CTLA-4 or (B) irrelevant transfectants and eGFP was stained with Human Anti-Human CTLA-4 (Research Grade Ipilimumab) Monoclonal Antibody (Catalog # MAB9928) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTLA-4

Ipilimumab is a biosimilar directed against the extracellular domain of CTLA‑4. This product constitutes the immunogen used for the anti-Idiotype catalog number MAB10064. The biotinylated antibody (catalog number FAB9928B) can be used in flow cytometry or various assay format to measure CTLA-4 expression and can be detected by conjugated streptavidin.

Long Name
Cytotoxic T-lymphocyte-associated Molecule 4
Entrez Gene IDs
1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
Alternate Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Product Datasheets

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FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  2. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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