Human CRACC/SLAMF7 Antibody

Catalog #: MAB19061 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19061-100
MAB19061-SP

Detection of CRACC/SLAMF7 in Human Tonsil.

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All CRACC/SLAMF7 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRACC/SLAMF7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CRACC/SLAMF7 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 235622

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRACC/SLAMF7
Lys27-Ser225
Accession # Q9NQ25

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of CRACC/SLAMF7 in Human Tonsil. CRACC/SLAMF7 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human CRACC/SLAMF7 Monoclonal Antibody (Catalog # MAB19061) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRACC/SLAMF7

CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8⁺ cells, CD4⁺ cells and B cells. CRACC may play a role in the activation and effector function of T cells and NK cells.

Entrez Gene IDs

57823 (Human); 75345 (Mouse); 102133710 (Cynomolgus Monkey)

Alternate Names

19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7