Human Complement Component C3aR PE-conjugated Antibody

Catalog #: FAB5995P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB5995P-100

Detection of C3a R in HEK293 Human Cell Line Transfected with Human C3a R and eGFP by Flow Cytometry.

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Human Complement Component C3aR PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Complement Component C3a R in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 534625

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human C3a R
Met1-Val482
Accession # Q16581

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of C3a R in HEK293 Human Cell Line Transfected with Human C3a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human C3a R and eGFP were stained with (A) Mouse Anti-Human C3aR PE-conjugated Monoclonal Antibody (Catalog # FAB5995P) or (B) Mouse IgG2A isotype control antibody (Catalog # IC003P).View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Complement Component C3aR

Long Name

Complement Component C3a Receptor

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Alternate Names

AZ3B; AZ3BC3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; C3AR1; C3ARcomplement component 3 receptor 1; C3R1; complement component 3a receptor 1; Complement Component C3a R; Complement Component C3aR; HNFAG09