Human Complement Component C1qA Antibody

Catalog #: MAB4035 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4035
MAB4035-SP

Detection of Complement Component C1qA in Human Placenta.

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Human Complement Component C1qA Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human Complement Component C1qA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Complement Component C1qB is observed.

Source

Monoclonal Rat IgG_2B Clone # 394107

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Complement Component C1qA
Lys110-Ala245
Accession # P02745

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Complement Component C1qA

Immunohistochemistry

1-15 µg/mL

Immersion fixed paraffin-embedded sections of Human Placenta

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Complement Component C1qA in Human Placenta. Complement Component C1qA was detected in immersion fixed paraffin-embedded sections of Human Placenta using Rat Anti-Human Complement Component C1qA Monoclonal Antibody (Catalog # MAB4035) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in cytotrophoblast in chorionic villi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1qA

C1qA, one of three subunits of complement component C1q, contains a collagen-like domain and a globular C1q domain. Within the globular domain, human C1qA shares 73% and 77% aa sequence identity with mouse and rat C1qA, respectively. Six disulfide-linked heterodimers of C1qA and C1qB associate with three disulfide-linked homodimers of C1qC to form C1q. Deficiency in C1qA is associated with the development of autoimmunity.

Long Name

Complement Component 1q Subcomponent, A Chain

Entrez Gene IDs

712 (Human); 12259 (Mouse); 298566 (Rat)

Alternate Names

C1QA complement component 1, q subcomponent, A chain; C1QA; Complement Component C1qA