Human CLEC3B/Tetranectin Antibody

Catalog #: MAB5170 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5170
MAB5170-SP

Detection of Human CLEC3B/Tetranectin by Western Blot.

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CLEC3B/Tetranectin Antibody Summary

Species Reactivity

Human

Specificity

Detects human CLEC3B/Tetranectin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 805035

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC3B/Tetranectin
Glu22-Val202
Accession # P05452

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 5-25 μg/mL of this antibody will block 50% of the binding of 10 μg/mL of Recombinant Human CLEC3B to immobilized Recombinant Human HGF coated at 5 μg/mL (100 μL/well). At 50 μg/mL, this antibody will block >90% of the binding

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human CLEC3B/Tetranectin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC3B/Tetranectin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human CLEC3B/Tetranectin by Simple Western^TM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. Specific bands were detected for CLEC3B/Tetranectin at approximately 32 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC3B/Tetranectin

CLEC3B (C-type lectin domain family 3-member B; also Tetranectin) is a 20 kDa, secreted member of the C type lectin superfamily. It is produced by multiple cell types including monocytes, neutrophils, fibroblasts, hepatocytes and various endocrine cells. Although named Tetranectin, CLEC3B is actually a nondisulfide linked homotrimer. It binds to plasminogen, sulfated polysaccharides, and fibrin. Human CLEC3B precursor is 202 amino acids (aa) in length. It contains a signal sequence (aa 1-21), a coiled coil region that mediates trimerization (aa 26-52) and a C type lectin domain (aa 77-198). CLEC3B contains O-linked glycosylation. Mature human CLEC3B is 81% aa identical to mouse CLEC3B.

Long Name

C-type Lectin-like Receptor 3B

Entrez Gene IDs

7123 (Human); 21922 (Mouse)

Alternate Names

CLEC3B; C-type lectin domain family 3 member B; C-type lectin domain family 3, member B; DKFZp686H17246; Plasminogen kringle 4-binding protein; TETN; tetranectin (plasminogen binding protein); Tetranectin; TNA; TNAtetranectin (plasminogen-binding protein); TNtetranectin