Human CLEC-2A Antibody Summary
Trp49-Leu174 (predicted)
Accession # Q6UVW9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human CLEC-2A antibody by Western Blot. Detection of Human CLEC-2A antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/CLEC-2A_MAB72191_Western_Blot_11811.jpg)
Detection of Human CLEC-2A by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC-2A at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![CLEC-2A antibody in Human Skin by Immunohistochemistry (IHC-P). CLEC-2A antibody in Human Skin by Immunohistochemistry (IHC-P).](https://resources.rndsystems.com/images/datasheets/antibody/CLEC-2A_MAB72191_Immunohistochemistry_11741.jpg)
CLEC-2A in Human Skin. CLEC-2A was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC-2A
CLEC-2A (C-type lectin domain family 2 member A), also known as PILAR and KACL, is an approximately 35 kDa type II transmembrane glycoprotein in the C-type lectin superfamily. It contains one C-type lectin domain in its extracellular region. Alternate splicing of human CLEC-2A generates an additional isoform with a substitution of 37 amino acids (aa) at the C-terminus. CLEC-2A is upregulated on antigen-activated CD8+ T cells and enhances T cell proliferation through interactions with CD161. It is also expressed on keratinocytes and binds to NKp65/KLRF2, triggering NK cell activation.
Product Datasheets
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