Human Choline Acetyltransferase/ChAT Antibody Summary
Ala2-Pro630
Accession # NP_066266
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Choline Acetyltransferase/ChAT in Human Brain. Choline Acetyltransferase/ChAT was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human Choline Acetyltransferase/ChAT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3447) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Choline Acetyltransferase/ChAT
ChAT catalyzes the production of the neurotransmitter acetylcholine (Ach), which is required for cholinergic neuron communication. It serves as a marker for functional cholinergic neurons in the central and peripheral nervous systems. At least six ChAT mRNAs encoding 69 kDa, 82 kDa and 74 kDa ChAT proteins have been identified. Compared to the 82 kDa form, 69 kDa ChAT lacks the N-terminal 118 amino acid extension containing a nuclear localization signal. As a result the 69 kDa ChAT is primarily localized to the cytoplasm. Human 69 kDa ChAT shares 86% amino acid sequence identity with the mouse or rat ChAT.
Product Datasheets
Citations for Human Choline Acetyltransferase/ChAT Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Loss of TMEM106B and PGRN leads to severe lysosomal abnormalities and neurodegeneration in mice
Authors: Feng T, Mai S, Roscoe JM et al.
EMBO Rep.
-
AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106bGrn mice
Authors: Feng T, Minevich G, Liu P et al.
iScience
-
Notch signaling regulates motor neuron differentiation of human embryonic stem cells.
Authors: Ben-Shushan E, Feldman E, Reubinoff B
Stem Cells, 2015-02-01;33(2):403-15.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Neural differentiation of embryonic stem cells induced by conditioned medium from neural stem cell.
Authors: Zhang JQ, Yu XB, Ma BF, Yu WH, Zhang AX, Huang G, Mao FF, Zhang XM, Wang ZC, Li SN, Lahn BT, Xiang AP
Neuroreport, 2006-07-17;17(10):981-6.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Choline Acetyltransferase/ChAT Antibody
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Human Choline Acetyltransferase/ChAT Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
1:4000 dilution on brain sections pretreated with 1% NaBH4.
Primary ab dilution 1:4000 (better with 1:2000)
40 ug protein per lane
