Human CETP Antibody

Name: Human CETP Antibody
Brand: R&D Systems
SKU: MAB10042
Rating: 5 (1 reviews)

Catalog #: MAB10042 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10042-100
MAB10042-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CETP Antibody Summary

Species Reactivity

Human

Specificity

Detects human CETP in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 995234

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CETP
Cys18-Ser493
Accession # P11597

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CETP in Human Liver. CETP was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CETP Monoclonal Antibody (Catalog # MAB10042) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CETP

Cholesteryl Ester Transfer Protein (CETP), also known as plasma lipid transfer protein, is 493 amino acids (aa) secreted plasma protein involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. CETP allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL.

Long Name

Cholesteryl Ester Transfer Protein

Entrez Gene IDs

1071 (Human)

Alternate Names

BPIFF; CETP; cholesteryl ester transfer protein; cholesteryl ester transfer protein, plasma; HDLCQ10; Lipid Transfer Protein I