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Human CD47 APC-conjugated Antibody

Catalog #: FAB4670A
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB4670A
Detection of CD47 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD47 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD47 in flow cytometry. Stains human CD47 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 472603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD47
Gln19-Pro139
Accession # Q08722
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD47 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD47 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 APC-conjugated Monoclonal Antibody (Catalog # FAB4670A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD47

CD47, also known as Integrin-associated Protein (IAP), is a ubiquitous 50 kDa multipass transmembrane protein with a single V-type Ig-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. It also binds to VEGFR2, inducing VEGFR2 signaling in both T cells and endothelial cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternative splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.

Entrez Gene IDs
961 (Human); 16423 (Mouse); 397042 (Porcine); 478552 (Canine); 102139846 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen

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Citations for Human CD47 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cultivation and characterization of pterygium as an ex vivo study model for disease and therapy
    Authors: N Josifovska, DJ Szabó, R Nagymihály, Z Veréb, A Facskó, K Eriksen, MC Moe, G Petrovski
    Cont Lens Anterior Eye, 2017-05-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
    Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
    PLoS ONE, 2012-10-09;7(10):e47187.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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