Human CD40/TNFRSF5 PE-conjugated Antibody

Catalog #: FAB6321P
2 Citations Datasheet / COA / SDS

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FAB6321P

Detection of CD40/TNFRSF5 in Human Blood Lymphocytes by Flow Cytometry.

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All CD40/TNFRSF5 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD40/TNFRSF5 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40.

Source

Monoclonal Mouse IgG_2B Clone # 82111

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5
Glu21-Arg193
Accession # P25942

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD40/TNFRSF5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE-conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD40/TNFRSF5 in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD40/TNFRSF5 PE‑conjugated Monoclonal Antibody (Catalog # FAB6321P) or (B) isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kD type I transmembrane glycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is 257 amino acids (aa) in length, and consists of a 173 aa extracellular domain (ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECD contains four cysteine-rich repeats, the second of which is considered crucial to ligand binding. Over aa 21-193, human CD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells known to constitutively or inducibly express CD40 are large in number and include basal layer keratinocytes, adipocytes, CD8⁺ T cells, fibrocytes (circulating stem cells), monocytes, eosinophils, dendritic cells, pancreatic duct epithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelial cells and B cells. There is at least one alternative splice form that shows a 38 aa substitution for aa 166-277, generating a soluble molecule. There are two "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, a spirochete that causes Lyme disease. CD40 ligation by CD154 has many outcomes, some of which are cell-specific. Ligation of CD40 on B cells (with IL-4) induces IgE production, while bone marrow stromal cell activation via CD40 promotes OPG secretion with concomitant bone formation. In addition, stimulation of CD40 on keratinocytes results in IL-8 and RANTES expression with subsequent leukocyte infiltration, while adipocyte activation via CD40 promotes local M1 polarization.

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Alternate Names

B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human CD40/TNFRSF5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
Species: Human
Sample Types: Whole Cells
Applications: Chip Cytometry
Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor.
Authors: Chiang CL, Ledermann JA, Aitkens E, Benjamin E, Katz DR, Chain BM
Clin. Cancer Res., 2008-08-01;14(15):4898-907.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry