Human CD4 mFluor™ Violet 500-Conjugated Antibody

Catalog #: FAB3791MFV500 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3791MFV500-100

Detection of CD4 in PBMCs by Flow Cytometry.

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Human CD4 mFluor™ Violet 500-Conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 11830

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant Human CD4.
Extracellular Domain
Accession # P01730

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

mFluor Violet 500 SE (Excitation= 410 nm, Emission= 501 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

PBMCs with CD3 costain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD4 in PBMCs by Flow Cytometry. PBMCs were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD4 mFluor™ Violet 500-Conjugated Monoclonal Antibody (Catalog # FAB3791MFV500) or (B) unstained cells. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4