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Human CD3 epsilon Fluorescein-conjugated Antibody

Catalog #: FAB100F
8 Citations Datasheet / COA / SDS
Clone UCHT1 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB100F-100
FAB100F-025
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD3 epsilon Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Monoclonal Mouse IgG1 Clone # UCHT1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human thymocytes (1)
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD3e antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) or (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Human CD3 epsilon Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
    Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
    AIDS, 2010-02-20;24(4):491-502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Over-expression of receptor activator of nuclear factor-kappaB ligand (RANKL), inflammatory cytokines, and chemokines in periprosthetic osteolysis of loosened total hip arthroplasty.
    Authors: Wang CT, Lin YT, Chiang BL, Lee SS, Hou SM
    Biomaterials, 2009-09-25;31(1):77-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
    Authors: Markel G, Seidman R, Besser MJ
    PLoS ONE, 2009-05-19;4(5):e5597.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
    Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
    Clin. Exp. Immunol., 2007-10-09;150(3):567-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
    Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
    FASEB J., 2007-03-14;21(10):2442-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways.
    Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J
    J. Mol. Biol., 2006-12-28;367(1):25-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. CD4(+) CD25(+) cells in type 1 diabetic patients with other autoimmune manifestations
    Authors: Dalia S. Abd Elaziz, Mona H. Hafez, Nermeen M. Galal, Safa S. Meshaal, Aisha M. El Marsafy
    Journal of Advanced Research
  8. Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues
    Authors: ZHENXIN WANG, JIANFENG YANG, YANBO ZHU, YUN ZHU, BIN ZHANG, YINGHUI ZHOU
    Oncology Letters

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