Human CD35 APC-conjugated Antibody

Catalog #: FAB5748A Datasheet / COA / SDS

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FAB5748A

Detection of CD35 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD35 APC-conjugated Antibody Summary

Specificity

Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed.

Source

Monoclonal Mouse IgG₁ Clone # 594708

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD35
Gln42-Asp1971 (His1208Arg)
Accession # CAA68755

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD35 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CD35 APC-conjugated Monoclonal Antibody (Catalog # FAB5748A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD35

CD35, also known as Complement Receptor 1 (CR1), is a 220-300 kDa N-glycosylated member of the RCA (Regulators of Complement Activation) family of proteins. CD35 binds and internalizes particles and immune complexes that are opsonized with MBL or complement components C3b, C3i, C4b, or C1q. CD35 additionally protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases. The extracellular domain (ECD) of human CD35 contains 30 tandem SCR/SUSHI repeats. A soluble form of the CD35 ECD circulates in the serum. A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35. Cell surface CD35 is widely expressed on hematopoietic cells.

Entrez Gene IDs

1378 (Human)

Alternate Names

C3BR; CD35; complement component (3b/4b) receptor 1 (Knops blood group); CR1; KN