Human CD25/IL-2R alpha PE-conjugated Antibody Summary
Glu22-Cys213
Accession # P01589
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2R alpha
Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
Product Datasheets
Citations for Human CD25/IL-2R alpha PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
Nature Communications, 2022-06-03;13(1):3121.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effects of High Consumption of Vegetables on Clinical, Immunological, and Antioxidant Markers in Subjects at Risk of Cardiovascular Diseases
Authors: I Peluso, A Raguzzini, G Catasta, V Cammisotto, A Perrone, C Tomino, E Toti, M Serafini
Oxid Med Cell Longev, 2018-10-08;2018(0):5417165.
Species: Human
Sample Types: Whole Blood
Applications: Flow Cytometry -
Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
J Immunol, 2013-12-02;192(1):123-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
2011-05-19;6(5):e19988.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
VEGFR2 is selectively expressed by FOXP3high CD4+ Treg.
Authors: Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M
Eur. J. Immunol., 2010-01-01;40(1):197-203.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
Authors: Ni Z, Walcheck B
Clin. Immunol., 2009-08-08;133(2):257-64.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
FEBS Lett., 2007-03-01;581(7):1317-22.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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