Human CD25/IL-2R alpha Antibody

Catalog #: MAB1020
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1020
MAB1020-SP

IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody.

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD25/IL-2R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.

Source

Monoclonal Mouse IgG_2A Clone # 24212

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
Glu22-Cys213
Accession # P01589

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD25/IL‑2 R alpha Fc Chimera (Catalog # 1020-RL)

Flow Cytometry

0.25 µg/10⁶ cells

Human Treg cells (PBMC treated overnight with anti-human CD3 (10 μg/mL; Catalog # MAB100), anti-human CD28 (5 μg/mL; Catalog # MAB342), TGF-beta (10 ng/mL; Catalog # 100-B), and rhIL-2 (20 ng/mL; Catalog # 202-IL).

Blockade of Receptor-ligand Interaction

0.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Blockade of Receptor-ligand Interaction

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IL-2 binding to Human CD25/IL-2R alpha-transfected HEK293 Human Cell Line is Blocked by Human CD25/IL-2R alpha Antibody. In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD25/IL-2R alpha

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Long Name

Interleukin 2 Receptor alpha

Entrez Gene IDs

3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)

Alternate Names

CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD25/IL-2R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
Nature Communications, 2022-06-03;13(1):3121.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion.
Authors: McEwen A, Maher M, Mo R, Gottardi C
Mol Biol Cell, 2014-06-25;25(16):2365-74.
Species: Canine
Sample Types: Whole Cells
Applications: ICC
A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties.
Authors: Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Tasken K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF
Eur. J. Immunol., 2010-01-01;40(1):134-41.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Authors: Ebert EC, Mehta V
Cell. Immunol., 2006-08-17;241(1):7-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation.
Authors: Voo KS, Peng G, Guo Z, Fu T, Li Y, Frappier L, Wang RF
Cancer Res., 2005-02-15;65(4):1577-86.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry