Human CD200R1 APC-conjugated Antibody

Catalog #: FAB3414A Datasheet / COA / SDS

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FAB3414A

Detection of CD200 R1 in Human Blood Monocytes by Flow Cytometry.

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All CD200R1 Antibodies

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Human CD200R1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD200 R1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 380525

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD200 R1
Ala27-Leu266 (predicted)
Accession # NP_620161

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD200 R1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD200 R1 APC-conjugated Monoclonal Antibody (Catalog # FAB3414A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CD200R1 in HEL-92 vs Jurkat cells by Flow Cytometry HEL-92 (filled histogram) and Jurkat cells (open histogram) were stained with Mouse Anti-Human CD200R1 APC‑conjugated Monoclonal Antibody (Catalog # FAB3414A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily (1‑3). The standard human CD200 R1 cDNA encodes a 325 amino acid (aa) precursor that includes a 28 aa signal sequence, a 215 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 61 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, human CD200 R1 shares 56% aa sequence identity with mouse and rat CD200 R1. Alternate splicing of the human CD200 R1 mRNA generates four isoforms, two of which are truncated in the Ig-C2 domain and are likely secreted. The protein expressed here contains a mature region that is identical to that of the standard form. There is an N-terminal extension of 25 aa that, in the standard form, is part of the signal sequence. In human, a separate CD200 R12 gene encodes a protein that shares 81% ECD aa identity with CD200 R11. In mouse, at least four genes for CD200 R1-like molecules have been described (4‑6). CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (7‑9), while its ligand, CD200, is widely distributed (10). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (11). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (12). The capacity of CD200 R1-like molecules to interact with CD200 is controversial (5, 13). CD200 R1 propagates inhibitory signals despite its lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (8, 9, 14, 15) CD200 R1-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 6).

References

Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
Wright, G.J. et al. (2003) J. Immunol. 171:3034.
Hatherley, D. et al. (2005) J. Immunol. 175:2469.
Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
Shiratori, I. et al. (2005) J. Immunol. 175:4441.
Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
Fallarino, F. et al. (2004) J. Immunol. 173:3748.
Wright, G.J. et al. (2001) Immunology 102:173.
Hoek, R.M. et al. (2000) Science 290:1768.
Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
Zhang, S. et al. (2004) J. Immunol. 173:6786.

Long Name

CD200 Receptor 1

Entrez Gene IDs

131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

Alternate Names

CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor