Human CD20 Antibody

Detection of CD20 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™ CD20 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD20 Monoclonal Antibody (MAB42252) at 3ug/mL dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of B-cells. Protocol available in COMET™ Panel Builder.

Detection of Human CD20 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for CD20 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD20 in Human PBMCs by Flow Cytometry. Human PBMCs were stained with (A) Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) or (B) Rabbit IgG Isotype Control Antibody (MAB1050), followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-associated Proteins protocol.

CD20 in Human Tonsil. CD20 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human CD20 by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD20 at approximately 42 kDa (as indicated) using 25 µg/mL of Rabbit Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42252). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.