Human CD163 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB16072S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB16072S-100UG
R&D Systems Antibodies
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Human CD163 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD163 in Flow Cytometry.
Source
Monoclonal Mouse IgG1 Clone # R20
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Balb/c mice immunized with Synovial cells from Rheumatoid Arthritis patients
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood monocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References
  1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Moller, H.J. et al. (2002) Blood 99:378.
  7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
  8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
  9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  15. Fabriek, B.O. et al. (2007) Blood 109:5223.
Entrez Gene IDs
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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