Human CD161 Antibody

Catalog #: MAB7448 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7448
MAB7448-SP

Detection of Human CD161 in Human Lymphocytes

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD161 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD161 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 702228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human CD161
Accession # Q12918

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Human CD161 antibody in Human Lymphocytes

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Detection of Human CD161 in Human Lymphocytes Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse IgG2B Isotype control (Catalog # MAB0041) or (B) Mouse Anti-Human CD161 Monoclonal Antibody (Catalog # MAB7448) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD161

CD161, also known as NKR-P1A and KLRB1, is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-g secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161⁺ cell populations are depleted in ulcerative colitis, Grave’s disease, and AIDS, although CD161⁺ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR‑P1B and D, and the stimulatory NKR‑P1A, C, and F. Human CD161 shares 47% aa sequence identity with mouse and rat NKR-P1A.

Entrez Gene IDs

3820 (Human); 25192 (Rat)

Alternate Names

CD161 antigen; CD161; CLEC5B; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; KLRB1; Ly59; Natural killer cell surface protein P1A; NKR; NKRP1; NKR-P1; NKRP1A; NKR-P1AMGC138614; NKRP1ANKR