Human CD11c Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1777N-100
FAB1777N-025
Detection of CD11c in Human PBMCs by Flow Cytometry.
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Human CD11c Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha X/CD11c in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # ICRF 3.9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rheumatoid synovial fluid cells and human monocyte-derived fibronectin
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD11c antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD11c in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CD11c Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1777N) or (B) Mouse IgG1Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
    12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: CD11c

Integrin alpha X, also known as CD11c, heterodimerizes with Integrin beta 2, also known as CD18. The CD11c/CD18 complex binds fibrinogen and has been reported to be a receptor for iC3b (1-3).

References
  1. Hogg, N. et al. (1986) Eur. J. Immunol. 16(3):240.
  2. Knapp, W.B. et al. eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York.
  3. Stacker, S.A. and T.A. Springer, J. Immunol. (1991) 146(2):648.
Entrez Gene IDs
3687 (Human); 16411 (Mouse); 64350 (Rat)
Alternate Names
95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human CD11c Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Aerobic exercise in humans mobilizes HSC in an intensity dependent manner
    Authors: Gianni Parise
    J. Appl. Physiol., 2016-11-23;0(0):jap.00696.201.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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