Human Cathepsin E Biotinylated Antibody

Catalog #: BAF1294 Datasheet / COA / SDS

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BAF1294

All Cathepsin E Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin E Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cathepsin E in Western blots. In Western blots, approximately 50% cross‑reactivity with recombinant mouse Cathepsin E is observed and less than 1% cross-reactivity with recombinant human (rh) Cathepsin D and rhBACE is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin E
Ile54-Pro396
Accession # NP_001901

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cathepsin E (Catalog # 1294-AS)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human skin and tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑17), a propeptide (residues 18‑53), and a mature chain (residues 54‑396) (4).

Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Alternate Names

CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase