Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody

Catalog #: BAF1049 Datasheet / COA / SDS

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BAF1049

All Cathepsin A/Lysosomal Carboxypeptidase A Antibodies

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Human Cathepsin A/Lysosomal Carboxypeptidase A Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cathepsin A/Lysosomal Carboxypeptidase A in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse Cathepsin A is observed and less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S, and rhCathepsin X/Z/P is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin A/Lysosomal Carboxypeptidase A
Ala29-Tyr480
Accession # P10619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cathepsin A/Lysosomal Carboxypeptidase A (Catalog # 1049-SE)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human prostate subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH. Also known as protective protein, its association with beta -galactosidase ( beta -gal) and neuraminidase is essential for beta -gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes the lysosomal storage disorder galactosialidosis, characterized by a combined secondary deficiency of beta -gal and neuraminidase. Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including tachykinins, indicating that extralysosomal Cathepsin A plays a role in regulation of functions of these molecules (2). Cathepsin A is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds.

Entrez Gene IDs

5476 (Human); 19025 (Mouse)

Alternate Names

beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; cathepsin ANGBE; CTSA; EC 3.4.16; EC 3.4.16.5; GLB2; GSL; Lysosomal Carboxypeptidase A; lysosomal protective protein; PPCA; PPGBprotective protein for beta-galactosidase (galactosialidosis); Protective protein cathepsin A; Protective protein for beta-galactosidase