Human Caspase-8 Antibody

Catalog # Availability Size / Price Qty
MAB704
MAB704-SP
Detection of Human Caspase‑8 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)

Human Caspase-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspase-8 precursor in Western blots and a 42 kDa doublet generated during apoptosis.
Source
Monoclonal Mouse IgG2A Clone # 84131
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human Caspase-8
aa 234-391
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Caspase‑8 antibody by Western Blot. View Larger

Detection of Human Caspase‑8 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-8 Monoclonal Antibody (Catalog # MAB704), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Caspase-8 at approximately 55 and 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-8

Caspase-8 (Cysteine-aspartic acid protease 8/Casp8; also MCH5 and FLICA) is a 28 kDa member of the peptidase C14A family of enzymes. It is known as the initiating caspase for the apoptotic cascade. Caspase-8 acts on procaspases-3, 4, 6, 7, 9 and 10, in addition to c-FLIPL and procaspase-8 itself. Human procaspase-8 alpha 4 is a 58 kDa (predicted), 496 amino acid (aa) protein and contains two N-terminal death domains plus a catalytic site that utilizes His334Gly335 plus Cys377. Following death domain-containing receptor activation, proteolysis generates a 28 kDa heterodimer. This includes a p18 subunit (aa 234-391; or 217-374 of the 8a/standard form) noncovalently linked to a p10 subunit (aa 402-496; or 385-479 of the 8a/standard form). Association with another p18/p10 heterodimer generates active caspase-8. There are two common procaspase-8 isoform variants. One 53 kDa isoform (8b) shows a deletion of aa 103-134, while a second 55 kDa (8a) isoform shows a deletion of aa 103-134 with a 15 aa insertion after Lys183. The p18 and p10 subunits are 68% and 82% aa identical, human to mouse, respectively.

Entrez Gene IDs
841 (Human); 12370 (Mouse)
Alternate Names
AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy

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Citations for Human Caspase-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis
    Authors: J Hou, R Zhao, W Xia, CW Chang, Y You, JM Hsu, L Nie, Y Chen, YC Wang, C Liu, WJ Wang, Y Wu, B Ke, JL Hsu, K Huang, Z Ye, Y Yang, X Xia, Y Li, CW Li, B Shao, JA Tainer, MC Hung
    Nat. Cell Biol., 2020-09-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Silencing of heat shock protein 27 increases the radiosensitivity of non?small cell lung carcinoma cells
    Authors: L Xu, X Lin, Y Zheng, H Zhou
    Mol Med Rep, 2019-05-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Regulation of the extrinsic apoptotic pathway by the extracellular matrix glycoprotein EMILIN2.
    Authors: Mongiat M, Ligresti G, Marastoni S, Lorenzon E, Doliana R, Colombatti A
    Mol. Cell. Biol., 2007-08-13;27(20):7176-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Enhanced susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in oral squamous cell carcinoma cells treated with phosphatidylinositol 3-kinase inhibitors.
    Authors: Uchida M, Iwase M, Takaoka S, Yoshiba S, Kondo G, Watanabe H, Ohashi M, Nagumo M, Shintani S
    Int. J. Oncol., 2007-05-01;30(5):1163-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
    Authors: Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
    J. Cell. Sci., 2006-09-26;119(0):4247-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human Caspase-8 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: A549 human lung carcinoma cell line,MBA-MB-123 human breast cancer cell line Species: Human