Human Carbohydrate Sulfotransferase 4/CHST4 Antibody

Catalog #: AF5357 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5357
AF5357-SP

Detection of Human Carbohydrate Sulfotransferase 4/CHST4 by Western Blot.

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All Carbohydrate Sulfotransferase 4/CHST4 Antibodies

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Human Carbohydrate Sulfotransferase 4/CHST4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse CHST4 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and rhCHST6 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Sulfotransferase 4/CHST4
Leu22-Gln286
Accession # Q8NCG5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 (Catalog # 5357-ST), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 (1.33 µg/mL, Catalog # 5357-ST) transfer from the sulfate donor Adenosine 3'-phosphate 5'-phosphosulfate (PAPS, 42 μM) to beta ‑D‑GlcNAc‑(1→6)-alpha -D-Man→OMe (833 μM). The Neutralization Dose (ND₅₀) is typically 10.7 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carbohydrate Sulfotransferase 4/CHST4 by Western Blot. Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5357) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 4/CHST4

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycan (5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.

References

deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278:40282.
Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
Uchimura, K. et al. (2002) J. Biol. Chem. 277:3979.
Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
MacRae, I.J. I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39:1613.
Wu, Z.L. et al. (2002) Faseb J. 16:539.

Entrez Gene IDs

10164 (Human); 26887 (Mouse); 307838 (Rat)

Alternate Names

carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; Carbohydrate Sulfotransferase 4; CHST4; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.17; EC 2.8.2.21; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; gn6st-2; GST3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; HEC-GLCNAC-6-ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; LSSTcarbohydrate sulfotransferase 4; N-acetylglucosamine 6-O-sulfotransferase 2