Human Carbohydrate Sulfotransferase 15/CHST15 Antibody

Catalog #: MAB3365 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3365
MAB3365-SP

All Carbohydrate Sulfotransferase 15/CHST15 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbohydrate Sulfotransferase 15/CHST15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbohydrate Sulfotransferase 15/CHST15 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 388421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Sulfotransferase 15/CHST15
Ser99-Thr561
Accession # Q7LFX5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Carbohydrate Sulfotransferase 15/CHST15 (Catalog # 3365-ST)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 15/CHST15

N-Acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST or CHST15) is a type II transmembrane protein composed of a short N‑terminal cytoplasmic domain and a long C-terminal luminal catalytic domain. CHST15 transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the 6-O of GalNAc-4S on chondroitin sulfate A (CS-A) as well as dermatan sulfate (1), generating the chondroitin sulfate E (CS-E) disaccharide unit GlcA beta 1-3GalNAc-4S,6S. CS-E binds with strong affinity to Midkine, a heparan sulfate-binding growth factor (2), playing an important regulatory role in differentiation and morphogenesis during embryonic development. In situ hybridization for the expression of CHST15 in the postnatal mouse brain showed a widespread expression of the transcript in the developing brain except at postnatal day 7 (3). For comparison, chondroitin sulfate 6-O sulfotransferase-1 (C6ST-1) encoded by the CHST3 gene, only recognizes and transfers sulfate to the 6-O of unsulfated GalNAc residues on chondroitin sulfate generating the chondroitin sulfate C (CS-C) disaccharide unit GlcA beta 1-3GalNAc-6S (4). The sequence of the human CHST15 was found to be the same as human B-cell RAG-associated protein (BRAG) (1).

References

Ohtake, S. et al. (2001) J. Biol. Chem. 276:43894.
Zou, P. et al. (2003) Glycobiology 13:35.
Purushothaman, A. et al. (2007) J. Biol. Chem. 282:19442.
Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10155.
Robbins, P.W. (1962) Methods in Enzymol. 5:964.
MacRae, I.J. et al. (2000) Biochemistry 39:1613.
Wu, Z.L. et al. (2002) FASEB J. 16:539.

Entrez Gene IDs

51363 (Human); 286974 (Rat)

Alternate Names

B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; BRAG; BRAGKIAA0598RP11-47G11.1; carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15; Carbohydrate Sulfotransferase 15; CHST15; DKFZp781H1369; EC 2.8.2.33; GALNAC4S6ST; GalNAc4S-6ST; hBRAG; MGC34346; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase