Human c-Myc PE-conjugated Antibody

Catalog #: IC3696P
7 Citations Datasheet / COA / SDS

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IC3696P

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.

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All c-Myc Antibodies

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human c-Myc PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 9E10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of c-Myc antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc PE-conjugated Monoclonal Antibody (Catalog # IC3696P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Entrez Gene IDs

4609 (Human); 17869 (Mouse); 24577 (Rat)

Alternate Names

avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human c-Myc PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Inducible Gene Switches with Memory in Human T Cells for Cellular Immunotherapy
Authors: Deboki Chakravarti, Leidy D. Caraballo, Benjamin H. Weinberg, Wilson W. Wong
ACS Synthetic Biology
Mechanistic modelling of tyrosine recombination reveals key parameters determining the performance of a CAR T cell switching circuit
Authors: Jack E. Bowyer, Deboki Chakravarti, Wilson W. Wong, Declan G. Bates
Engineering Biology
Lipid accumulation-mediated histone hypoacetylation drives persistent NK cell dysfunction in anti-tumor immunity
Authors: Jiao, D;Sun, R;Ren, X;Wang, Y;Tian, P;Wang, Y;Yuan, D;Yue, X;Wu, Z;Li, C;Gao, L;Ma, C;Liang, X;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
J. Biol. Chem., 2020-08-19;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
"Inflammescent" CX3CR1+CD57+ CD8 T cells are generated and expanded by IL-15
Authors: SR Morris, B Chen, JC Mudd, S Panigrahi, CL Shive, SF Sieg, CM Cameron, DA Zidar, NT Funderburg, SA Younes, B Rodriguez, S Gianella, MM Lederman, ML Freeman
JCI Insight, 2020-06-04;0(0):.
Species: Human
Sample Types: Whole Cells
Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila
Authors: A Pandey, BM Harvey, MF Lopez, A Ito, RS Haltiwange, H Jafar-Neja
Cell Rep, 2019-11-12;29(7):2054-2066.e6.
Species: Drosophila melanogaster
Sample Types: Whole Cell
Applications: Flow Cytometry
Abnormal Expression of c-Myc Oncogene in NK Cells in Patients with Cancer
Authors: GK Zakiryanov, E Kustova, NT Urazalieva, ET Baimuchame, NN Nakisbekov, MR Shurin
Int J Mol Sci, 2019-02-11;20(3):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry