Human Brg1 Antibody Summary
Gln673-Asn774
Accession # P51532
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Brg1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, cytoplasmic and nuclear extracts. PVDF membrane was probed with 2 µg/mL Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Brg1 was detected at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Brg1 in HeLa Human Cell Line. Brg1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Brg1 in Rat Cortical Stem Cells. Brg1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Western Blot Shows Human Brg1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Brg1 knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Goat Anti-Human Brg1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5738) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Brg1 at approximately 250 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Brg1
Brg1 (Brm/Swi2-related gene 1), also known as SMARCA4 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 4), SNF2L4 and SNF2 beta, is a 205 kDa member of the SNF2 helicase family of molecules. It is a ubiquitously expressed, nuclear-localized chromatin remodeling ATPase that, depending upon its associated complex, may both facilitate and inhibit gene transcription. Human SMARCA4 is 1647 amino acids (aa) in length, and contains an HSA domain (aa 460‑532), one BRK region (aa 610‑654), six potential phosphoserine sites between aa 610‑662, a DEXDc domain that unwinds RNA and DNA (aa 774‑913), one Bromo domain (aa 1477‑1547) and eight potential phosphoserine sites between aa 1570‑1644.
Product Datasheets
Citations for Human Brg1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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The Pdx1 Bound Swi/Snf Chromatin Remodeling Complex Regulates Pancreatic Progenitor Cell Proliferation and Mature Islet beta Cell Function
Authors: Spaeth JM, Liu JH, Peters D et al.
Diabetes
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Divergent transcriptional regulation of astrocyte reactivity across disorders
Authors: JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew
Nature, 2022-05-25;0(0):.
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Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes
Authors: R.L. Maher, C.G. Marsden, A.M. Averill, S.S. Wallace, J.B. Pederson, D.S. Pederson
DNA Repair (Amst.)
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