Human Brevican Antibody

Catalog #: MAB4009 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4009
MAB4009-SP

All Brevican Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Brevican Antibody Summary

Species Reactivity

Human

Specificity

Detects human Brevican in direct ELISAs and Western blots. Also detects a truncated version of recombinant human Brevican (aa 23‑649).

Source

Monoclonal Mouse IgG₁ Clone # 450613

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Brevican isoform 1
Asp23-Pro911
Accession # AAH09117

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Brevican (Catalog # 4009-BC) under non-reducing conditions only

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Brevican (Catalog # 4009-BC), see our available Western blot detection antibodies

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.01-0.05 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan (132 kDa) to immobilized Recombinant Human Brevican (Catalog # 4009-BC) coated at 3 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brevican

Brevican, also called BEHAB, is a secreted member of the the lectican family of proteoglycans that share a common domain structure (1). Brevican contains an Ig‑like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (2). Brevican is restricted to the CNS and is expressed by astrocytes, oligodendrocytes, and neurons (3‑7). A GPI-anchored alternate splice form exists that is truncated following the central (GAG) region (2, 8). An additional isoform results from an alternate start site at Met228. Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (5, 9, 10). Full-length brevican consists of a 97 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (4, 7, 11, 12). Brevican associates with the extracellular matrix, perineuronal nets, and astrocyte cell surfaces by means of its chondroitin sulfate, GPI anchor, hyaluronic acid-binding link domains, and the core protein (4, 7, 8, 13). The secreted isoform is dominant during brain development and is upregulated in astrocytes following brain injury (2, 14). In human and rat, an under-glycosylated form of brevican is upregulated in highly aggressive glioma but not in low-grade glioma or other brain pathologies (15, 16). In mouse and rat, levels of an ADAMTS4-generated 55 kDa N‑terminal fragment increase during remodeling after excitotoxic injury (11, 12). Human brevican shares 90%, 80%, and 80% aa sequence identity with bovine, mouse, and rat brevican, respectively. Within the Ig‑like and two link domains, brevican shares 45%‑51% aa sequence identity with aggrecan, neurocan, and versican.

References

Viapiano, M.S. and R.T. Matthews (2006) Trends Mol. Med. 12:488.
Gary, S.C. et al. (2000) Gene 256:139.
Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495.
Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206.
Hamel, M.G. et al. (2005) J. Neurochem. 93:1533.
Ogawa, T. et al. (2001) J. Comp. Neurol. 432:285
Yamada, H. et al. (1997) J. Neurosci. 17:7784.
Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
Mayer, J. et al. (2005) BMC Neurosci. 6:52.
Yuan, W. et al. (2002) Neuroscience 114:1091.
Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
Jaworski, D.M. et al. (1999) Exp. Neurol. 157:327.
Viapiano, M.S. et al. (2005) Cancer Res. 65:6726.
Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.

Long Name

Brain Specific Proteoglycan in the Aggrecan Family

Entrez Gene IDs

63827 (Human); 12032 (Mouse); 25393 (Rat)

Alternate Names

ALPBRE; BCAN; BEHAB; BEHABbrevican core protein; Brain-enriched hyaluronan-binding protein; Brevican; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB; chondroitin sulfate proteoglycan BEHAB/brevican; CSPG7; CSPG7brevican proteoglycan; MGC13038