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Human BMP-4 Antibody

Catalog #: AF757
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF757
AF757-SP
Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) BMP‑2 and 10% cross-reactivity with rhBMP-5 and rhBMP-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BMP‑4 (Catalog # 314-BP)
Neutralization
Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line [Nakamura, K. et al. (1999) Exp. Cell Res. 250:351]. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 15 ng/mL Recombinant Human BMP‑4 and 2 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP-4 (Catalog # 314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF757). The ND50 is typically 0.5-2.0 µg/mL in the presence of heparin (2 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 - 5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer (7 - 9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR‑IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13 - 15).

 

References
  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

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Citations for Human BMP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Bone morphogenetic protein-2-induced Wnt/beta-catenin signaling pathway activation through enhanced low-density-lipoprotein receptor-related protein 5 catabolic activity contributes to hypertrophy in osteoarthritic chondrocytes
    Authors: Ioanna Papathanasiou, Konstantinos N Malizos, Aspasia Tsezou
    Arthritis Research & Therapy
  2. Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration
    Authors: I Wagner, H Wang, PM Weissert, WL Straube, A Shevchenko, M Gentzel, G Brito, A Tazaki, C Oliveira, T Sugiura, A Shevchenko, A Simon, DN Drechsel, EM Tanaka
    Dev. Cell, 2017-03-27;40(6):608-617.e6.
    Applications: Immunoprecipitation, Western Blot
  3. A novel, visible light-induced, rapidly cross-linkable gelatin scaffold for osteochondral tissue engineering.
    Authors: Mazaki T, Shiozaki Y, Yamane K, Yoshida A, Nakamura M, Yoshida Y, Zhou D, Kitajima T, Tanaka M, Ito Y, Ozaki T, Matsukawa A
    Sci Rep, 2014-03-25;4(0):4457.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals.
    Authors: Pollett JB, Corsi KA, Weiss KR, Cooper GM, Barry DA, Gharaibeh B, Huard J
    Stem Cells, 2007-06-14;25(9):2302-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
    Authors: Maeshima A, Vaughn DA, Choi Y, Nigam SK
    Dev. Biol., 2006-04-27;295(2):473-85.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Neutralization
  6. VEGF-A signaling through Flk-1 is a critical facilitator of early embryonic lung epithelial to endothelial crosstalk and branching morphogenesis.
    Authors: Del Moral PM, Sala FG, Tefft D, Shi W, Keshet E, Bellusci S, Warburton D
    Dev. Biol., 2005-12-22;290(1):177-88.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. The Dose of Growth Factors Influences the Synergistic Effect of Vascular Endothelial Growth Factor on Bone Morphogenetic Protein 4–Induced Ectopic Bone Formation
    Authors: Guangheng Li, Karin Corsi-Payne, Bo Zheng, Arvydas Usas, Hairong Peng, Johnny Huard
    Tissue Engineering Part A
  8. FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing
    Authors: Régis P. Lemaitre, Aliona Bogdanova, Barbara Borgonovo, Jeffrey B. Woodruff, David N. Drechsel
    BMC Biotechnology

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