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Human BMI-1 Antibody

Catalog #: MAB33342
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB33342
MAB33342-SP
BMI‑1 in Human Breast Cancer Tissue.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMI-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMI-1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 384509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry BMI‑1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

BMI‑1 in Human Breast Cancer Tissue. BMI‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BMI‑1 Monoclonal Antibody (Catalog # MAB33342) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry BMI-1 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

BMI‑1 in Human Breast. BMI-1 was detected in immersion fixed paraffin-embedded sections of normal human breast using Human BMI-1 Monoclonal Antibody (Catalog # MAB33342) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMI-1

BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 is 99%, 97%, 99% and 99% aa identical to bovine, mouse, feline and canine BMI-1, respectively.

Long Name
B Lymphoma Mo-MLV Insertion Region 1
Entrez Gene IDs
648 (Human); 12151 (Mouse)
Alternate Names
B lymphoma Mo-MLV insertion region 1 homolog (mouse); B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1; BMI-1; FLVI2/BMI1; MGC12685; murine leukemia viral (bmi-1) oncogene homolog; PCGF4; polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; RING finger protein 51; RNF51; RNF51Polycomb group RING finger protein 4

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Citations for Human BMI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Bmi-1 in gallbladder carcinoma: Clinicopathology and mechanism of regulation of human gallbladder carcinoma proliferation
    Authors: Kai Jiao, Weijiao Jiang, Chunyang Zhao, Dewang Su, Haomin Zhang
    Oncology Letters
  2. Lymphocyte to monocyte ratio predicts survival and is epigenetically linked to miR-222-3p and miR-26b-5p in diffuse large B cell lymphoma
    Authors: AM Metwally, AAHM Kasem, MI Youssif, SM Hassan, AHA Abdel Waha, LA Refaat
    Scientific Reports, 2023-03-25;13(1):4899.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis
    Authors: L Wu, D Zhang, L Zhou, Y Pei, Y Zhuang, W Cui, J Chen
    EBioMedicine, 2019-02-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  4. p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs.
    Authors: Chang CJ, Chao CH, Xia W, Yang JY, Xiong Y, Li CW, Yu WH, Rehman SK, Hsu JL, Lee HH, Liu M, Chen CT, Yu D, Hung MC
    Nat. Cell Biol., 2011-02-20;13(3):317-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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