Human BLAME/SLAMF8 Biotinylated Antibody

Catalog #: BAM19074 Datasheet / COA / SDS

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BAM19074

All BLAME/SLAMF8 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BLAME/SLAMF8 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BLAME/SLAMF8 in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 250008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BLAME/SLAMF8
Ala23-Asp233
Accession # Q9P0V8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Human BLAME/SLAMF8 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human BLAME/SLAMF8 Antibody (Catalog # MAB19073)

Standard: Recombinant Human BLAME/SLAMF8 Protein, CF (Catalog # 1907-BL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). Mature human BLAME consists of a 211 amino acid (aa) ECD that contains two Ig V-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares 19%‑26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN-gamma stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3).

References

McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.

Long Name

SLAM Family Member 8

Entrez Gene IDs

56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)

Alternate Names

B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8