Human BATF3 Antibody

Catalog #: AF7437
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7437
AF7437-SP

Detection of Human BATF3 by Western Blot.

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All BATF3 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BATF3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BATF3 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse BATF3 is observed, and less than 2% cross-reactivity with recombinant human BATF is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BATF3
Met1-Arg127
Accession # Q9NR55

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BATF3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BATF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7437) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BATF3 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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BATF3 in HDLM‑2 Human Cell Line. BATF3 was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Sheep Anti-Human BATF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7437) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BATF3

BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c⁺), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.

Long Name

Basic Leucine Zipper Transcription Factor, ATF-like 3

Entrez Gene IDs

55509 (Human); 381319 (Mouse); 60462 (Rat)

Alternate Names

basic leucine zipper transcription factor, ATF-like 3; basic leucine zipper transcriptional factor ATF-like 3; BATF3; B-ATF-3; FLJ36352; FLJ37535,21 kDa small nuclear factor isolated from T-cells; JDP1; Jun dimerization protein 1; Jun dimerization protein p21SNFT; JUNDM1; SNFT; SNFT21-kD small nuclear factor isolated from T cells

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human BATF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Super-enhancer-based identification of a BATF3/IL-2R-module reveals vulnerabilities in anaplastic large cell lymphoma
Authors: HC Liang, M Costanza, N Prutsch, MW Zimmerman, E Gurnhofer, IA Montes-Moj, BJ Abraham, N Prokoph, S Stoiber, S Tangermann, C Lobello, J Oppelt, I Anagnostop, T Hielscher, S Pervez, W Klapper, F Zammarchi, DA Silva, KC Garcia, D Baker, M Janz, N Schleussne, F Fend, Š Pospíšilov, A Janiková, J Wallwitz, D Stoiber, I Simonitsch, L Cerroni, S Pileri, L de Leval, D Sibon, V Fataccioli, P Gaulard, C Assaf, F Knörr, C Damm-Welk, W Woessmann, SD Turner, AT Look, S Mathas, L Kenner, O Merkel
Nature Communications, 2021-09-22;12(1):5577.
Species: Human
Sample Types: Whole Cells
Applications: ChIP
Immunogenomic determinants of tumor microenvironment correlate with superior survival in high-risk neuroblastoma
Authors: Riyue Bao, Stefani Spranger, Kyle Hernandez, Yuanyuan Zha, Peter Pytel, Jason J Luke et al.
Journal for ImmunoTherapy of Cancer
Type I interferon-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors
Authors: U Govender, B Corre, Y Bourdache, S Pellegrini, F Michel
J. Leukoc. Biol, 2017-02-27;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot