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Human B7-H2 PerCP-conjugated Antibody

Catalog #: FAB165C
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB165C
Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry.
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Citations (2)
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Human B7-H2 PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 136726
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7-H2
Asp19-Ser258
Accession # O75144
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B7‑H2 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7-H2 PerCP-conjugated Monoclonal Antibody (Catalog # FAB165C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H2

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References
  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Ling, V. et al. (2000) J. Immunol. 164:1653.
  3. Wang, S. et al. (2000) Blood 96:2808.
  4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
  5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  6. Swallow, M.M. et al. (1999) Immunity 11:423.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
B7 Homolog 2
Entrez Gene IDs
23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
Alternate Names
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand

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Citations for Human B7-H2 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Enterovirus-infected beta-cells induce distinct response patterns in BDCA1+ and BDCA3+ human dendritic cells.
    Authors: Schulte B, Gielen P, Kers-Rebel E, Schreibelt G, van Kuppeveld F, Adema G
    PLoS ONE, 2015-03-25;10(3):e0121670.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Survival of Ovarian Cancer Patients Is Independent of the Presence of DC and T Cell Subsets in Ascites
    Authors: Christina Wefers, Tjitske Duiveman-de Boer, Refika Yigit, Petra L. M. Zusterzeel, Anne M. van Altena, Leon F. A. G. Massuger et al.
    Frontiers in Immunology

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