Human B7-H2 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7-H2 PE-conjugated Monoclonal Antibody (Catalog # FAB165P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: B7-H2
Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with human and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7‑H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Ling, V. et al. (2000) J. Immunol. 164:1653.
- Wang, S. et al. (2000) Blood 96:2808.
- Brodie, D. et al. (2000) Curr. Biol. 10:333.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Swallow, M.M. et al. (1999) Immunity 11:423.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Product Datasheets
Citations for Human B7-H2 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Differential Modulation of Human M1 and M2 Macrophage Activity by ICOS-Mediated ICOSL Triggering
Authors: CL Gigliotti, C Dianzani, I Stoppa, C Monge, S Sutti, D Sblattero, C Puricelli, R Rolla, U Dianzani, E Boggio
International Journal of Molecular Sciences, 2023-02-02;24(3):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
B7h triggering inhibits the migration of tumor cell lines.
Authors: Dianzani C, Minelli R, Gigliotti C, Occhipinti S, Giovarelli M, Conti L, Boggio E, Shivakumar Y, Baldanzi G, Malacarne V, Orilieri E, Cappellano G, Fantozzi R, Sblattero D, Yagi J, Rojo J, Chiocchetti A, Dianzani U
J Immunol, 2014-04-11;192(10):4921-31.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100
