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Human ASC Antibody

Catalog #: AF3805
6 Citations Datasheet / COA / SDS
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AF3805
AF3805-SP
Detection of Human ASC by Western Blot.
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Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASC Antibody Summary

Species Reactivity
Human
Specificity
Detects human ASC in Western blots.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Knockout Validated
ASC is specifically detected in MCF‑7 human breast cancer parental cell line but is not detectable in ASC knockout MCF‑7 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human ASC antibody by Western Blot. View Larger

Detection of Human ASC by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western Detection of Human ASC antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human ASC by Simple WesternTM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and U937 human histiocytic lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ASC at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human ASC Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human ASC Specificity by Using Knockout Cell Line. Western blot shows lysates of MCF-7 human breast cancer parental cell line and ASC knockout MCF-7 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ASC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ASC at approximately 23 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: ASC

Apoptosis-associated speck-like protein containing a CARD (ASC) is also known as Target of Methylation-mediated Silencing (TMS-1) and PYD and CARD-domain containing protein (PYCARD). ASC is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli.

Long Name
Apoptosis-Associated Speck-Like Protein Containing A CARD
Entrez Gene IDs
29108 (Human)
Alternate Names
apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; PYCARD; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; TMS; TMS1; TMS-1

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Citations for Human ASC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Caspase-1 interdomain linker cleavage is required for pyroptosis
    Authors: Ball DP, Taabazuing CY, Griswold AR et al.
    Life Sci Alliance
  2. Downregulation of miR-137 Facilitates CD4+ T Cell Pyroptosis in Systemic Lupus Erythematosus via Stimulating AMPK Pathway
    Authors: A Gong, L Mi, F Wei, Y Zhuang, Y Song, C Pan, X Zhang, G Lin, Z Wu, Y Liu
    Journal of Immunology Research, 2023-02-09;2023(0):1241774.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome
    Authors: SD Rao, Q Chen, Q Wang, EL Orth-He, M Saoi, AR Griswold, A Bhattachar, DP Ball, HC Huang, AJ Chui, DJ Covelli, S You, JR Cross, DA Bachovchin
    Nature Chemical Biology, 2022-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
    Authors: Yana Liu, Shihong Zhang, Zhiwei Xue, Xiaoxia Zhou, Lin Tong, Jiachen Liao et al.
    Bioengineered
  5. Promoter Methylation Leads to Decreased ZFP36 Expression and Deregulated NLRP3 Inflammasome Activation in Psoriatic Fibroblasts
    Authors: Matteo Bertesi, Sebastian Fantini, Claudia Alecci, Roberta Lotti, Andrea Martello, Sandra Parenti et al.
    Frontiers in Medicine
  6. Magnesium Sulfate Mitigates the Progression of Monocrotaline Pulmonary Hypertension in Rats
    Authors: CY Chang, HJ Shih, IT Huang, PS Tsai, KY Chen, CJ Huang
    Int J Mol Sci, 2019-09-18;20(18):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P

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